Subgram daily supplementation with docosahexaenoic acid protects low-density lipoproteins from oxidation in healthy men
Subgram daily supplementation with docosahexaenoic acid protects low-density lipoproteins from oxidation in healthy men
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DOI：
10.1016/j.atherosclerosis.2009.07.049
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发表时间：
2010-02-01
期刊：
影响因子：
5.3
通讯作者：
Lagarde, Michel
中科院分区：
文献类型：
--
作者：
Calzada, Catherine;Colas, Romain;Lagarde, Michel
目的：为了确定增加剂量的二十二碳六烯酸（DHA）作为唯一的n-3多不饱和脂肪酸（PUFA）补充剂对低密度脂蛋白（LDL）氧化还原状态和氧化能力的影响结果：即使在补充200 mg/天DHA两周后，LDL磷脂和胆固醇酯中DHA的比例也呈剂量依赖性增加。每日摄入200、400和800 mg DHA导致α-生育酚浓度增加，MDA浓度降低，铜诱导的LDL氧化滞后时间延长。补充1600毫克/天的DHA对上述参数没有影响。在血浆中，4-羟基-己烯醛的浓度，特别是来自n-3脂肪酸的过氧化，在800和1600 mg DHA后显著增加，占血浆n-3 PUFA的0.01%，而4-羟基-壬烯醛的浓度，来自n-6脂肪酸的过氧化，没有变化。我们的研究结果清楚地表明，摄入200-800毫克/天的DHA可能对LDL具有保护和抗氧化作用，并可能代表健康人群预防心血管疾病的最佳剂量。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Objective: To determine the effect of supplementation with increasing doses of docosahexaenoic acid (DHA), as the only n-3 polyunsaturated fatty acid (PUFA), on low-density lipoprotein (LDL) redox status and oxidizability.Methods: Twelve healthy men aged 53-65 years ingested consecutive doses of DHA (200, 400, 800 and 1600 mg/day), each dose for two weeks.Results: The proportions of DHA increased dose-dependently in LDL phospholipids and cholesteryl esters, even after two weeks of supplementation with 200 mg/day DHA. The daily intake of 200, 400 and 800 mg DHA resulted in increased alpha-tocopherol concentrations, decreased MDA concentrations, and a longer lag time for copper-induced LDL oxidation. Supplementation with 1600 mg/day DHA had no effect on the above parameters. In plasma, concentrations of 4-hydroxy-hexenal, specifically derived from the peroxidation of n-3 fatty acids, significanty increased after 800 and 1600 mg DHA, representing 0.01% of plasma n-3 PUFAs, while 4-hydroxy-nonenal concentrations, derived from the peroxidation of n-6 fatty acids, did not change.Conclusion: Our results clearly show that an intake of 200-800 mg/day DHA may have protective and antioxidant effects on LDL and could represent optimal doses for cardiovascular disease prevention in a healthy population. (C) 2009 Elsevier Ireland Ltd. All rights reserved.