Mechanostimulation, electrostimulation and force measurement in an in vitro model of the isolated rat diaphragm
Mechanostimulation, electrostimulation and force measurement in an in vitro model of the isolated rat diaphragm
复制标题
离体大鼠隔膜体外模型中的机械刺激、电刺激和力测量
DOI：
--
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发表时间：
2011
影响因子：
3.2
通讯作者：
S. Schumann
中科院分区：
文献类型：
--
作者：
C. Armbruster;C. Dassow;K. Gamerdinger;Matthias Schneider;M. Šumkauskaitė;J. Guttmann;S. Schumann
在整个大鼠膈肌的体外模型中，研究了在限定的预负荷水平下的肌肉收缩力。共24个离体大鼠膈肌内的两室应变施加器进行电刺激。通过测量隔膜下方腔室中的诱发压力，在八个调整的预载水平上确定所产生的收缩力。随后，横膈膜暴露于四种处理之一6小时：（1）对照，（2）周期性机械拉伸，（3）间歇性电刺激或（4）周期性机械拉伸和电刺激的组合。膈肌收缩力从最低前负荷水平的116 ± 21 mN增加到最大前负荷水平的775 ± 85 mN。6小时后，非电刺激处理后最大肌肉收缩力最小（对照：81 ± 15 mN，机械偏转：94 ± 12 mN），电刺激处理后最大（单纯电刺激：165 ± 20 mN，机械和电刺激联合：164 ± 14 mN）。我们的结论是，我们的模型允许孤立的大鼠横膈膜上的力测量。此外，我们的结论是，通过中间电刺激的反作用力产生更好地保存比机械刺激。
In an in vitro model of the entire rat diaphragm, diaphragmatic contraction forces at defined preload levels were investigated. A total of 24 excised rat diaphragms were electrically stimulated inside a two-chamber strain-applicator. The resulting contraction forces were determined on eight adjusted preload levels via measuring the elicited pressure in the chamber below the diaphragm. Subsequently, diaphragms were exposed for 6 h to one of four treatments: (1) control, (2) cyclic mechanical stretch, (3) intermittent electrical stimulation or (4) combination of cyclic mechanical stretch and electrical stimulation. Diaphragmatic contraction force increased from 116 ± 21 mN at the lowest preload level to 775 ± 85 mN at the maximal preload level. After 6 h maximal muscle contraction forces were smallest after non-electrostimulated treatment (control: 81 ± 15 mN, mechanical deflection: 94 ± 12 mN) and largest after electrostimulation treatment (mere electrostimulation: 165 ± 20 mN, combined mechano- and electro-stimulation: 164 ± 14 mN). We conclude that our model allows force measurements on isolated rat diaphragms. Furthermore, we conclude that by intermediate electrical stimulation diaphragmatic force generation was better preserved than by mechanical stimulation.
弹性蛋白酶诱导的肺气肿对膈肌产生力的能力的影响。
DOI：
10.1172/jci110709
发表时间：
1982
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Supinski,GS;Kelsen,SG
通讯作者：
Kelsen,SG
影响因子：
3.3
作者：
Sassoon, CSH;Caiozzo, VJ;Sieck, GC
通讯作者：
Sieck, GC