LIQUID-CHROMATOGRAPHY IN OPEN-TUBULAR COLUMNS - THEORY OF COLUMN OPTIMIZATION WITH LIMITED PRESSURE AND ANALYSIS TIME, AND FABRICATION OF CHEMICALLY BONDED REVERSED-PHASE COLUMNS ON ETCHED BOROSILICATE GLASS-CAPILLARIES
LIQUID-CHROMATOGRAPHY IN OPEN-TUBULAR COLUMNS - THEORY OF COLUMN OPTIMIZATION WITH LIMITED PRESSURE AND ANALYSIS TIME, AND FABRICATION OF CHEMICALLY BONDED REVERSED-PHASE COLUMNS ON ETCHED BOROSILICATE GLASS-CAPILLARIES
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DOI：
10.1016/s0021-9673(01)88293-5
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
GUTHRIE, EJ
中科院分区：
文献类型：
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作者：
JORGENSON, JW;GUTHRIE, EJ
提出了一种理论，该理论表明，如果对有效压力和分析时间加以限制，开管柱将具有明确定义的最佳直径长度。结果表明，最佳直径对操作压力和分析时间的选择不敏感，在很宽的条件下介于1~3μm之间。十八烷基硅烷被化学键合到这些粗糙的表面上，柱以反相模式运行。溶质的检测是用柱上荧光检测器。
A theory is developed which shows that open-tubular columns will have well defined optimum diameters lengths if limits are placed on the available pressure and analysis time. The optimum diameters are shown to be rather insensitive to choice of operating pressure and analysis time, and lie between 1 and 3μm for a wide range of conditions.A method of etching borosilicate glass capillaries to increase surface roughness and column capacity factors is described. Octadecylsilane is chemically bonded to these roughened surfaces and columns are operated in a reversed-phase mode. Detection of solutes is with an on-column fluorescence detector.