Photochemical regulation of the activity of an endonuclease BamHI using an azobenzene moiety incorporated site-selectively into the dimer interface
Photochemical regulation of the activity of an endonuclease BamHI using an azobenzene moiety incorporated site-selectively into the dimer interface
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DOI：
10.1039/b409844g
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发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Majima, T
中科院分区：
文献类型：
--
作者：
Nakayama, K;Endo, M;Majima, T
合成了在二聚体界面（azoAla-BamHI）中具有偶氮苯丙氨酸残基的核酸内切酶BamHI突变体;虽然使用反式-azoAla-BamHI几乎抑制了活性，但用光照射产生的顺式异构体恢复了其固有活性。
Endonuclease BamHI mutants having an azophenylalanine residue in the dimer interface (azoAla-BamHI) were synthesized; while the activity was almost suppressed using trans-azoAla-BamHI, the cis-isomer generated with photoirradiation recovered its intrinsic activity.