Berberine attenuates cardiac dysfunction in hyperglycemic and hypercholesterolemic rats
Berberine attenuates cardiac dysfunction in hyperglycemic and hypercholesterolemic rats
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DOI：
10.1016/j.ejphar.2011.03.024
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发表时间：
2011-06-25
影响因子：
5
通讯作者：
Sun, Jian-Ning
中科院分区：
文献类型：
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作者：
Dong, Shi-Fen;Hong, Ying;Sun, Jian-Ning
黄连素(30 mg/kg/d，i.g.在高血糖和高胆固醇血症大鼠模型上评价其对心功能不全的影响。高糖高脂饲料(HSFD)由20%蔗糖、10%猪油、2.5%胆固醇、I%胆盐和链脲佐菌素(30 mg/kg)组成，连续12周诱发高血糖和高胆固醇血症。与对照组相比，HSFD/链脲佐菌素治疗组大鼠的血糖、总胆固醇和甘油三酯水平显著升高(分别为422%、194%和82%)。黄连素治疗可使高血糖高胆固醇血症大鼠的血糖和血脂水平降低24-69%。高血糖/高胆固醇血症大鼠的心输出量、左心室收缩压、心肌收缩最大速率(+dp/dtmax)、左室舒张末压和心肌最大舒张期最大速率(-dp/dtmax)等心功能受损16~55%。黄连素使高血糖、高胆固醇血症大鼠的心输出量、左心室收缩压和+dp/dtmax分别增加16%和79%，使左室舒张末压和-dp/dtmax分别降低121%和61%。黄连素使高血糖/高胆固醇血症大鼠心肌脂肪酸转运蛋白-1(159%)、脂肪酸转运蛋白(56%)、脂肪酸β-氧化酶(52%)以及葡萄糖转运蛋白-4(GT4)和过氧化物体增殖物激活受体-γ(PPAR-γ)的表达显著增加，而PPARα的mRNA和蛋白表达显著降低。提示黄连素对高血糖/高胆固醇血症所致的心功能不全有保护作用，其机制可能是通过减少心脏脂质堆积和促进葡萄糖转运来实现的。(C)2011爱思唯尔B.V.保留所有权利。
The positive effects of berberine (30 mg/kg/day, i.g. for 6 weeks) on cardiac dysfunction were evaluated in the rat model of hyperglycemia and hypercholesterolemia. Hyperglycemia and hypercholesterolemia were induced by feeding high-sucrose/fat diet (HSFD) consisting of 20% sucrose, 10% lard, 2.5% cholesterol, I% bile salt for 12 weeks and streptozotocin (30 mg/kg, i.p.). The plasma sugar, total cholesterol, and triglyceride levels were significantly increased (422, 194 and 82%, respectively) in the HSFD/streptozotocin-treated rats, when compared with control animals receiving normal diet and vehicle. Berberine treatment reduced the plasma sugar and lipid levels by 24-69% in the rat model of hyperglycemia and hypercholesterolemia. Cardiac functions signed as values of cardiac output, left ventricular systolic pressure, the maximum rate of myocardial contraction (+ dp/dtmax), left ventricular end diastolic pressure and the maximum rate of myocardial diastole (-dp/dt max) were injured by 16-55% in the hyperglycemic/hypercholesterolemic rats. Berberine increased cardiac output, left ventricular systolic pressure and + dp/dtmax by 64, 16 and 79%, but decreased left ventricular end diastolic pressure and -dp/dtmax by 121 and 61% in the rats receiving HSFD/streptozotocin, respectively, when compared with the drug-untreated rats of hyperglycemia and hypercholesterolemia. Berberine caused significant increase in cardiac fatty acid transport protein-1 (159%), fatty acid transport proteins (56%), fatty acid beta-oxidase (52%), as well as glucose transporter-4 and peroxisome proliferator-activated receptor-gamma (PPAR gamma), but decrease in PPAR alpha mRNA and protein expression in hyperglycemic/hypercholesterolemic rats. These results indicated that berberine exerted protective effects on cardiac dysfunction induced by hyperglycemia/hypercholesterolemia through alleviating cardiac lipid accumulation and promoting glucose transport. (C) 2011 Elsevier B.V. All rights reserved.