Effects of pH on human bone marrow stromal cells in vitro:: Implications for tissue engineering of bone
Effects of pH on human bone marrow stromal cells in vitro:: Implications for tissue engineering of bone
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DOI：
10.1002/jbm.10050
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发表时间：
2002-05-01
期刊：
影响因子：
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通讯作者：
Krebsbach, PH
中科院分区：
文献类型：
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作者：
Kohn, DH;Sarmadi, M;Krebsbach, PH
本研究的目的是解决这一假设，即细胞外pH值的变化改变胶原基因表达，胶原合成，碱性磷酸酶活性在骨髓基质细胞（BMSCs）。pH对细胞功能的潜在影响对于组织工程特别重要，因为相当大的努力被放在工程可生物降解的聚合物上，这些聚合物在降解时可能产生局部酸性微环境。以2 × 104个细胞/cm 2的密度接种人和鼠单细胞骨髓悬液。培养7天后，将培养基的pH调节至六个范围之一：大于或等于7.8、7.5-7.7、7.2-7.4、6.9-7.1、6.6-6.8或小于或等于6.5。48小时后，暴露于改变pH值，碱性磷酸酶活性和胶原蛋白的合成显着降低pH值。这种下降是两到三倍的pH值从7.5降低到6.6。相比之下，α 1（I）前胶原mRNA水平增加2 - 3倍，pH值降低。骨钙素mRNA的表达趋势与胶原相反。细胞外pH值的微小变化导致BMSCs表达成骨细胞表型标志物的能力发生显著变化。这些pH效应可能与组织工程支架提供的微环境有关，并表明降解聚合物支架可能会影响细胞在直接环境中的生物活性。(C)2002年约翰威利父子公司
The objective of this study was to address the hypothesis that changes in extracellular pH alter collagen gene expression, collagen synthesis, and alkaline phosphatase activity in bone marrow stromal cells (BMSCs). Potential effects of pH on cell function are of particular importance for tissue engineering because considerable effort is being placed on engineering biodegradable polymers that may generate a local acidic microenvironment on degradation. Human and murine single-cell marrow suspensions were plated at a density of 2 x 10(4) cells/cm(2). After 7 days in culture, the pH of the culture medium was adjusted to one of six ranges: greater than or equal to7.8, 7.5-7.7, 7.2-7.4, 6.9-7.1, 6.6-6.8, or less than or equal to6.5. After 48 h of exposure to an altered pH, alkaline phosphatase activity and collagen synthesis decreased significantly with decreasing pH. This decrease was two-to threefold as pH decreased from 7.5 to 6.6. In contrast, alpha1(I) procollagen mRNA levels increased two- to threefold as pH was decreased. The trend in osteocalcin mRNA expression was opposite to that of collagen. Small shifts in extracellular pH led to significant changes in the ability of BMSCs to express markers of the osteoblast phenotype. These pH effects potentially relate to the microenvironment supplied by a tissue-engineering scaffold and suggest that degrading polymer scaffolds may influence the biologic activity of the cells in the immediate environment. (C) 2002 John Wiley Sons, Inc.