DNA Damage Products (5′R)- and (5′S)-8,5′-Cyclo-2′-deoxyadenosines as Potential Biomarkers in Human Urine for Atherosclerosis
DNA Damage Products (5′R)- and (5′S)-8,5′-Cyclo-2′-deoxyadenosines as Potential Biomarkers in Human Urine for Atherosclerosis
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DOI：
10.1021/bi201912c
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发表时间：
2012-03-06
期刊：
影响因子：
2.9
通讯作者：
Dizdaroglu, Miral
中科院分区：
文献类型：
--
作者：
Jaruga, Pawel;Rozalski, Rafal;Dizdaroglu, Miral
我们假设 DNA 损伤产物 (5'R)-8,5'-环-2'-脱氧腺苷 (R-cdA) 和 (5'S)-8,5'-环-2'-脱氧腺苷 (S-cdA) 可能是非常适合动脉粥样硬化风险和诊断的生物标志物。我们通过使用同位素稀释液相色谱串联质谱法测量动脉粥样硬化患者和健康个体尿液中 R-cdA 和 S-cdA 以及另一种产物 8-羟基-2'-脱氧鸟苷 (8-OH-dG) 的水平来检验这一假设。我们发现，动脉粥样硬化患者尿液中这些产品的浓度明显高于健康人。我们的数据表明 R-cdA 和 S-cdA。可以在人尿液中准确且可重复地测量其作为动脉粥样硬化风险和诊断的潜在生物标志物。
We hypothesized that DNA damage products (5'R)-8,5'-cyclo-2'-deoxyadenosine (R-cdA) and (5'S)-8,5'-cyclo-2'-deoxyadenosine (S-cdA) may be well-suited biomarkers of risk and diagnosis for atherosclerosis. We tested this hypothesis by measuring the levels of R-cdA and S-cdA and another product, 8-hydroxy-2'-deoxyguanosine (8-OH-dG), in urine of atherosclerosis patients and healthy individuals using liquid chromatography tandem mass spectrometry with isotope dilution. We showed the presence of these products at significantly greater concentrations in urine of atherosclerosis patients than in that of healthy individuals. Our data suggest that R-cdA and S-cdA. cart be accurately and reproducibly measured in human urine as potential biomarkers of risk and diagnosis for atherosclerosis.