Cleavage-based signal amplification of RNA
Cleavage-based signal amplification of RNA
复制标题
基于 RNA 切割的信号放大
DOI：
10.1038/ncomms2492
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发表时间：
2013-02-01
影响因子：
16.6
通讯作者：
Tang, Zhuo
中科院分区：
文献类型：
--
作者：
Zhao, Yongyun;Zhou, Li;Tang, Zhuo
RNA检测已成为当前生物医学研究的一个组成部分。迄今为止，反转录-PCR是检测mRNA靶点最实用的方法。然而，通过逆转录-PCR进行RNA检测需要复杂的设备，并且对基因组DNA的污染高度敏感。在这里，我们报告了一种新的等温反应，同时扩增和检测RNA，基于切割的DNAzyme和信号放大。基于切割的RNA信号扩增不会被基因组DNA污染，适用于mRNA和microRNA靶标的检测，具有高特异性和灵敏度。此外，检测结果可以以比色或实时荧光的方式报告，用于不同的检测目的。
RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme and signal amplification. Cleavage-based signal amplification of RNA cannot be contaminated by genomic DNA and is suitable for the detection of both mRNA and microRNA targets, with high specificity and sensitivity. Moreover, the detection results can be reported in a colorimetric or real-time fluorometric way for different detection purposes.