Germline transgenesis of the ascidian Ciona intestinalis by electroporation
Germline transgenesis of the ascidian Ciona intestinalis by electroporation
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DOI：
10.1002/gene.20096
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发表时间：
2005-02-01
期刊：
影响因子：
1.5
通讯作者：
Sasakura, Y
中科院分区：
文献类型：
--
作者：
Matsuoka, T;Awazu, S;Sasakura, Y
Minos转座子的显微注射是唯一报道的在世界性海鞘中产生稳定的转基因系的技术。为了建立一个更适合的方法来产生稳定的转基因玻璃海鞘，我们研究了使用电穿孔的DNA到鸡蛋的可能性。0-44.4%的电穿孔个体将转基因传递给下一代。转基因整合到一条染色体上，多个拷贝的转基因插入到染色体的一个位点，表明电穿孔是一种简单而有效的技术，用于实现稳定的转基因在C。好吧与可能的内陆培养海鞘一起，该技术将用于产生在特定组织或器官中具有报告基因表达的稳定系，以及产生在插入位置中含有大量Minos转座子的转座酶表达稳定的转基因（启动子）系和突变体系。（C）2005 Wiley-Liss，Inc.
Microinjection of the Minos transposon is the only reported technique for generating stable transgenic lines in the cosmopolitan ascidian, Ciona intestinalis. To establish a more amenable method for generating stable transgenic Ciona, we examined the possibility of using electroporation of DNA into eggs. From 0-44.4% of electroporated individuals transmitted transgenes to the next generation. The transgene was integrated into one chromosome and multiple copies of the transgene were inserted into one site of the chromosome, indicating that electroporation is an easy and powerful technique for achieving stable transgenesis in C. intestinalis. Together with possible inland culture of this ascidian, this technique will be useful for generating stable lines which have reporter gene expression in a specific tissue or organ and the generation of transposase-expressing stable transgenic (jump-starter) lines and mutator lines which contain a lot of Minos transposons in an insertion position. genesis (C) 2005 Wiley-Liss, Inc.