Taurine‐Induced Attenuation of MPP+ Neurotoxicity In Vitro
Taurine‐Induced Attenuation of MPP+ Neurotoxicity In Vitro
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DOI：
10.1046/j.1471-4159.2000.0742087.x
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发表时间：
2000-05
影响因子：
4.7
通讯作者：
Muriel B. O'byrne;K. Tipton
中科院分区：
文献类型：
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作者：
Muriel B. O'byrne;K. Tipton
摘要：牛磺酸是一种含硫的β -氨基酸，在大脑和心脏等可兴奋组织中存在高浓度（毫摩尔）。它的作用包括渗透调节剂、温度调节剂、神经调节剂和潜在的神经递质。这种氨基酸也被证明在缺血和兴奋毒素诱导的神经元损伤期间大量释放。在这里，我们报道了牛磺酸对MPP+诱导的大鼠脑冠状动脉切片神经毒性的保护作用。在牛磺酸浓度为20和1 mM时观察到显著的保护作用，这表明牛磺酸在神经元损伤的情况下可能起作用。对合成牛磺酸类似物磷酸牛磺酸、胍乙烷磺酸和三甲基牛磺酸的研究表明，观察到的效果是通过细胞外机制介导的。GABA受体配体muscimol和bicuculline的使用表明这种作用是通过激活GABAA受体介导的。
Abstract: Taurine is a sulphur‐containing β‐amino acid found in high (millimolar) concentrations in excitable tissues such as brain and heart. Its suggested roles include osmoregulator, thermoregulator, neuromodulator, and potential neurotransmitter. This amino acid has also been shown to be released in large concentrations during ischaemia and excitotoxin‐induced neuronal damage. Here we report a protective effect of taurine against MPP+‐induced neurotoxicity in coronal slices from rat brain. Significant protective effects were observed at taurine concentrations of 20 and 1 mM, suggesting a potential role for taurine in cases of neuronal insult. Studies with the synthetic taurine analogues taurine phosphonate, guanidinoethane sulphonate, and trimethyltaurine suggested the observed effect to be mediated via an extracellular mechanism. The use of GABA receptor ligands muscimol and bicuculline indicated the effect to be mediated through activation of GABAA receptors.