Morphological changes in glial fibrillary acidic protein immunopositive astrocytes in the hippocampus of dietary-induced obese mice
Morphological changes in glial fibrillary acidic protein immunopositive astrocytes in the hippocampus of dietary-induced obese mice
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DOI：
10.1097/wnr.0000000000000180
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发表时间：
2014-08-06
期刊：
影响因子：
1.7
通讯作者：
Ruiz-Gayo, Mariano
中科院分区：
文献类型：
--
作者：
Cano, Victoria;Valladolid-Acebes, Ismael;Ruiz-Gayo, Mariano
长期食用高脂肪饮食（HFD）已被证明会引发代谢和心血管疾病。相比之下，这种类型的饮食制度对中枢神经系统，特别是下丘脑以外的影响，研究得很少。星形胶质细胞是脑中最丰富的神经胶质细胞群，在调节谷氨酸能传递中起关键作用，因为它们负责大部分谷氨酸的摄取和代谢。接受HFD的小鼠表现出学习和记忆的缺陷，以及与海马神经元能活动受损相容的神经化学和电生理变化。由于星形胶质细胞的功能和形态已被证明是相互依赖的，我们推测HFD是否会引发星形胶质细胞形态的变化。为此，我们使用了小鼠饮食诱导的肥胖模型。我们已经分析了星形胶质细胞的形态和密度，胶质细胞酸性蛋白免疫组化，以及谷氨酸转运蛋白，GLT-1（谷氨酸转运蛋白1型）和GLAST（星形胶质细胞谷氨酸转运蛋白），在海马CA 3区的表达。我们发现HFD小鼠的星形胶质细胞显示出更长和更少的投射。这些变化伴随着GLT-1和GLAST的上调。我们的数据表明，先前在HFD小鼠中检测到的功能障碍伴随着海马内的形态学变化。(C)2014年威科健康垂直酒吧利平科特威廉姆斯&威尔金斯。
Long-term consumption of a high-fat diet (HFD) has been shown to trigger both metabolic and cardiovascular diseases. In contrast, the effect of this type of dietary regime on the central nervous system, particularly outside the hypothalamus, has been investigated poorly. Astrocytes, the most abundant population of glial cells in the brain, are pivotal in regulating glutamatergic transmission as they are responsible for most of the glutamate uptake and metabolism. Mice on an HFD show deficits in learning and memory, together with neurochemical and electrophysiological changes compatible with the impairment in hippocampal glutamatergic activity. Because astrocyte function and morphology have been shown to be interdependent, we speculated whether HFD would trigger changes in astrocyte morphology. For this purpose, we have used a model of diet-induced obesity in mice. We have analyzed astrocyte morphology and density by glial fibrillary acidic protein immunohistochemistry, as well as the expression of the glutamate transporters, GLT-1 (glutamate transporter type-1), and GLAST (astrocyte glutamate transporter), in the CA3 area of the hippocampus. We found that astrocytes from HFD mice showed longer and less abundant projections. These changes were accompanied by the upregulation of both GLT-1 and GLAST. Our data show that the functional impairment detected previously in HFD mice is concomitant with morphological changes within the hippocampus. (C) 2014 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.