MAT alpha 1 can mediate gene activation by a-mating factor.
MAT alpha 1 can mediate gene activation by a-mating factor.
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MAT α 1 可以通过 a-交配因子介导基因激活。
DOI：
10.1101/gad.5.10.1924
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发表时间：
1991
影响因子：
10.5
通讯作者：
Cochran,BH
中科院分区：
文献类型：
--
作者：
Sengupta,P;Cochran,BH
在酿酒酵母中，α特异基因的表达受MATα1和MCM1基因产物的控制。MATα1和MCM1协同结合到阿尔法特异基因上游的PQ元件。PQ元件不仅指导α-特异表达，而且还可以指导基因诱导，以响应交配信息素的处理。我们使用基因融合来研究PQ盒的诱导是通过MATα1还是MCM1介导，还是两者的组合。当MCM1与细菌抑制子LexA的DNA结合区域融合时，该融合蛋白能够反式激活由LexA操纵子驱动的LacZ报告基因。然而，用a因子处理细胞并不能进一步增强MCM1-LexA融合的转录活性。MATα1-LexA融合蛋白也能够通过LexA操纵子反式激活。此外，α-因子还能进一步诱导MATα1-LexA融合蛋白的活性。当融合蛋白中MATα1的氨基末端逐渐缺失时，反式激活的基础水平逐渐降低，但融合蛋白的诱导性增加。MATα1-LexA融合蛋白在MATα1的氨基末端缺失57个氨基酸，不能反式激活。此外，MATα1-LexA融合蛋白的活性取决于编码信息素反应途径组成部分的STE7、STE11和STE12基因的功能。
In the yeast Saccharomyces cerevisiae, expression of alpha-specific genes is governed by the MAT alpha 1 and MCM1 gene products. MAT alpha 1 and MCM1 bind cooperatively to PQ elements upstream of alpha-specific genes. The PQ element not only directs alpha-specific expression but can also direct gene induction in response to treatment with a-mating pheromone. We have used gene fusions to investigate whether induction conferred by the PQ box is mediated through either MAT alpha 1 or MCM1, or a combination of both. When MCM1 is fused to the DNA-binding domain of the bacterial repressor LexA, this fusion protein is capable of trans-activating a lacZ reporter gene driven by a LexA operator. However, the transcriptional activity of the MCM1-LexA fusion is not further enhanced by treatment of cells with a-factor. A MAT alpha 1-LexA fusion protein is also capable of trans-activation through a LexA operator. Moreover, the activity of the MAT alpha 1-LexA fusion protein can be further induced by treatment with a-factor. When progressive deletions are made from the amino terminus of MAT alpha 1 in the fusion protein, the basal level of trans-activation progressively decreases, but the inducibility of the fusion protein increases. MAT alpha 1-LexA fusion proteins, which have greater than or equal to 57 amino acids deleted from the amino terminus of MAT alpha 1 are not capable of trans-activation. In addition, the activity of the MAT alpha 1-LexA fusion protein is dependent on the functions of the STE7, STE11, and STE12 genes that encode components of the pheromone response pathway.