A synthetic approach to structure-function relationships in the murine epidermal growth factor molecule.
A synthetic approach to structure-function relationships in the murine epidermal growth factor molecule.
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鼠表皮生长因子分子结构与功能关系的综合方法。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
R. B. Merrifield
中科院分区：
文献类型：
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作者：
W. Heath;R. B. Merrifield
鼠表皮生长因子是一种含有53个氨基酸的肽，对许多细胞类型具有有丝分裂作用，已通过固相法合成。合成肽在氨基酸组成、色谱行为、受体结合和DNA合成刺激方面与天然物质相同。构建了42- 53,32 -53和15-53残基对应的EGF分子片段，以及EGF的蛋氨酸亚砜衍生物[Met(O)21]EGF-(1-53)和EGF的聚合物形式。[Met(O)21]EGF-(1-53)在受体结合和刺激DNA合成方面的活性略低于EGF。聚合EGF的活性是EGF的1/100，而EGF-(15-53)的效力比EGF-(1-53)低10倍(4)。EGF-(32-53)活性更低，EGF-(43-53)活性更低。
Murine epidermal growth factor, a 53-amino acid peptide that is mitogenic for a number of cell types, has been synthesized by the solid-phase method. The synthetic peptide is identical to the natural material in amino acid composition, chromatographic behavior, receptor binding, and stimulation of DNA synthesis. Fragments of the EGF molecule corresponding to residues 42-53, 32-53, and 15-53 were constructed as well as the methionine sulfoxide derivative of EGF, [Met(O)21]EGF-(1-53), and a polymeric form of EGF. [Met(O)21]EGF-(1-53) was slightly less active than EGF in receptor binding and stimulation of DNA synthesis. Polymeric EGF was 1/100th as active as EGF, while EGF-(15-53) was less potent than EGF-(1-53) by a factor of 10(4). EGF-(32-53) was even less active and EGF-(43-53) was inactive.