Resonance assignments of a VapC family toxin from Clostridium thermocellum
Resonance assignments of a VapC family toxin from Clostridium thermocellum
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DOI：
10.1007/s12104-016-9702-y
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发表时间：
2016-07
影响因子：
0.9
通讯作者：
Chen Wang;Jinsong Xuan;Q. Cui;Yingang Feng
中科院分区：
文献类型：
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作者：
Chen Wang;Jinsong Xuan;Q. Cui;Yingang Feng
毒素-抗毒素（TA）系统广泛存在于细菌的质粒、噬菌体和染色体中，在细菌的生长持久性和宿主-病原体相互作用中起着重要作用。毒力相关蛋白BC （vir毒力相关蛋白BC， VapBC）家族TAs是细菌中最丰富的TAs，许多病原体的基因组中含有大量的VapBC基因座，已被广泛研究。Clostridium thermocellum是一种纤维素分解厌氧革兰氏阳性细菌，在生物燃料生产中有很好的应用前景，它的基因组中也含有一个VapBC TA。尽管已经确定了几个VapBC家族TAs的结构，但c的毒素和抗毒素成分。thermocellumVapBC与PDB中的蛋白序列同源性很低。因此，这种TA的结构和功能机制在很大程度上是未知的。在这里，我们报道了来自mc的VapC毒素的核磁共振定位。热细胞瘤为进一步的结构和功能研究奠定了基础。
Toxin–antitoxin (TA) systems widely exist in bacterial plasmids, phages, and chromosomes and play important roles in growth persistence and host-pathogen interaction. Virulence associated protein BC (VapBC) family TAs are the most abundant TAs in bacteria and many pathogens contain a large number ofvapBCloci in the genome which have been extensively studied.Clostridium thermocellum, a cellulolytic anaerobic gram-positive bacterium with promising applications in biofuel production, also contains a VapBC TA in the genome. Despite the structures of several VapBC family TAs have been determined, the toxin and anti-toxin components ofC. thermocellumVapBC have very low sequence identity to the proteins in PDB. Therefore, the structure and functional mechanism of this TA is largely unknown. Here we reported the NMR resonance assignments of the VapC toxin fromC. thermocellumas a basis for further structural and functional studies.