Use of Swinbourne plots to study potential suicide substrates: effects of ATP and ADP on yeast mitochondrial F1-ATPase.
Use of Swinbourne plots to study potential suicide substrates: effects of ATP and ADP on yeast mitochondrial F1-ATPase.
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使用 Swinbourne 图研究潜在的自杀底物：ATP 和 ADP 对酵母线粒体 F1-ATP 酶的影响。
DOI：
10.1016/0003-2697(85)90170-8
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发表时间：
1985
影响因子：
2.9
通讯作者：
Jenkins,WT
中科院分区：
文献类型：
--
作者：
Jenkins,WT
提出了一种简单的分析程序，用于比较酶在底物存在和不存在的情况下的失活率。发现催化反应过程中酵母 F1-ATP 酶的快速失活是由于某些阴离子而不是 ATP 或 ADP 造成的。 MgATP 未能保护酶，但用硫酸根、乙酸根、碳酸氢根或 N-三（羟甲基）甲基-2-氨基乙磺酸根阴离子取代并用 ADP 预孵育可防止失活。
A simple analytical procedure for comparing the rates of inactivation of an enzyme in the presence and absence of its substrate is proposed. The rapid inactivation of yeast F1-ATPase during the catalytic reaction was found to be due to certain anions rather than due to ATP or ADP. MgATP failed to protect the enzyme but substituting sulfate, acetate, bicarbonate, or N-tris(hydroxymethyl)methyl-2-aminoethane sulfonate anions and preincubation with ADP prevented the inactivation.