Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity.
Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity.
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表达双突变霍乱毒素 A 和 B 亚基的口服 MucoRice 可诱导毒素特异性中和免疫。
DOI：
10.1016/j.vaccine.2009.07.071
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发表时间：
2009
期刊：
影响因子：
5.5
通讯作者：
Kiyono,Hiroshi
中科院分区：
文献类型：
--
作者：
Yuki,Yoshikazu;Tokuhara,Daisuke;Nochi,Tomonori;Yasuda,Hiroshi;Mejima,Mio;Kurokawa,Shiho;Takahashi,Yuko;Kataoka,Nobuhiro;Nakanishi,Ushio;Hagiwara,Yukari;Fujihashi,Kohtaro;Takaiwa,Fumio;Kiyono,Hiroshi
水稻表达的霍乱毒素B亚单位（CT B）是一种无冷链的口服疫苗，能有效诱导中和肠毒素的免疫反应。我们创建了另一种基于大米的疫苗MucoRice，表达具有CTA和CTB亚基的无毒双突变霍乱毒素（dmCT）。Western-blot分析表明MucoRice-dmCT具有多组分疫苗的形状。口服MucoRice-dmCT诱导CTB特异性血清IgG和粘膜IgA抗体，而不是CTA特异性血清IgG和粘膜IgA抗体，产生针对霍乱毒素的保护性免疫，而不诱导大米蛋白特异性抗体应答。MucoRice-dmCT的效力与MucoRice-CTB疫苗的效力相同。MucoRice具有作为安全的多组分疫苗表达系统的潜力。
Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.
影响因子：
4.4
作者：
M. Marinaro;H. Staats;T. Hiroi;R. Jackson;M. Coste;P. Boyaka;N. Okahashi;M. Yamamoto;Hiroshi Kiyono;Horst Bluethmann;K. Fujihashi;Jerry R. McGhee
通讯作者：
M. Marinaro;H. Staats;T. Hiroi;R. Jackson;M. Coste;P. Boyaka;N. Okahashi;M. Yamamoto;Hiroshi Kiyono;Horst Bluethmann;K. Fujihashi;Jerry R. McGhee
影响因子：
13.8
作者：
Qu, LQ;Takaiwa, F
通讯作者：
Takaiwa, F
DOI：
10.1073/pnas.94.10.5267
发表时间：
1997-05-13
影响因子：
11.1
作者：
Yamamoto, S;Kiyono, H;McGhee, JR
通讯作者：
McGhee, JR