A nonsymbiotic hemoglobin gene from maize, ZmHb, is involved in response to submergence, high-salt and osmotic stresses
A nonsymbiotic hemoglobin gene from maize, ZmHb, is involved in response to submergence, high-salt and osmotic stresses
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来自玉米的非共生血红蛋白基因 ZmHb 参与对淹没、高盐和渗透胁迫的响应
DOI：
10.1007/s11240-008-9436-3
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发表时间：
2008-08
影响因子：
3
通讯作者：
Gu, Riliang
中科院分区：
文献类型：
--
作者：
Gao, Peng;Wang, Guoying;Zhao, Li;Gu, Riliang
非共生血红蛋白（nsHbs）参与植物的多种细胞过程。先前对nsHbs的研究表明它们的功能与缺氧应激有关。本文从玉米自交系宗31中克隆并鉴定了玉米nsHb基因（ZmHb）及其5 '侧链序列。南方分析表明，ZmHb基因存在于玉米基因组的两个拷贝或少量拷贝中。Northern blot表达分析表明，除了低氧胁迫外，玉米幼苗中ZmHb mRNA水平还受到高盐胁迫和渗透胁迫的诱导。启动子-GUS分析表明，ZmHb启动子驱动的GUS活性定位于根尖和维管组织。此外，在上述环境胁迫条件下，它也处于类似的基于mRNA水平的调控模式。ZmHb在转基因烟草中的异位表达增强了植物对淹水、盐度和渗透胁迫的耐受性。这些结果表明，ZmHb在多种胁迫条件下在玉米根系中转录水平上的表达受到调控，表明ZmHb在植物抗逆性中起重要作用。
Nonsymbiotic hemoglobins (nsHbs) are involved in a variety of cellular processes in plants. Previous studies on nsHbs suggest their function in response to hypoxic stress. Here, we report on the cloning and characterization of a maize nsHb gene (ZmHb) and its 5′ flanking sequences from maize inbred Zong31. Southern analysis suggests that the ZmHb gene is present in two copies or a low number of copies in the maize genome. Expression analysis by Northern blot shows that ZmHb mRNA levels in maize seedlings are induced by high-salt and osmotic stresses in addition to hypoxic stress. Promoter-GUS analysis has revealed that ZmHb promoter-driven GUS activity is localized to root tips and vascular tissues. Moreover, it is under similar patterns of regulation based on mRNA levels under the above environmental stress conditions. Ectopic expression of ZmHb in transgenic tobacco has enhanced plant tolerance to submergence, salinity and osmotic stresses. These results indicate that expression of ZmHb at transcript levels is regulated under multiple stress conditions in maize roots, suggesting an important role of ZmHb in plant stress tolerance.
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影响因子：
3.5
作者：
Csaba Seregélyes;A. Igamberdiev;A. Maassen;J. Hennig;D. Dudits;R. Hill
通讯作者：
Csaba Seregélyes;A. Igamberdiev;A. Maassen;J. Hennig;D. Dudits;R. Hill
影响因子：
3.9
作者：
Guldner, E;Godelle, B;Galtier, N
通讯作者：
Galtier, N
影响因子：
4.3
作者：
Dordas, C;Hasinoff, BB;Hill, RD
通讯作者：
Hill, RD
影响因子：
7.2
作者：
Dordas, C;Hasinoff, BB;Hill, RD
通讯作者：
Hill, RD
影响因子：
4.9
作者：
Qu, Zhan-Liang;Zhong, Nai-Qin;Xia, Gui-Xian
通讯作者：
Xia, Gui-Xian