Rapid diagnosis of tuberculosis by detection of mycobacterial lipoarabinomannan in urine
Rapid diagnosis of tuberculosis by detection of mycobacterial lipoarabinomannan in urine
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DOI：
10.1016/s0167-7012(01)00239-1
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发表时间：
2001-05-01
影响因子：
2.2
通讯作者：
Svenson, SB
中科院分区：
文献类型：
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作者：
Hamasur, B;Bruchfeld, J;Svenson, SB
迫切需要改进活动性结核病 (TB) 的实验室诊断工具。在这里，我们描述了两种方法，即追赶 ELISA 和基于尿液中阿拉伯脂甘露聚糖 (LAM) 检测的试纸测试。 LAM 是分枝杆菌外细胞壁的主要且特定的糖脂成分。初步实验表明，腹腔注射结核分枝杆菌粗细胞壁制剂的小鼠的尿液中会排泄 LAM。两种方法都高度灵敏，分别检测浓度为 1 ng/ml 和 5 pg/ml 的 LAM。 15 名活动性 IB 患者的尿液中均显示出中等到高水平的 LAM（吸光度值从 0.3 到 1.2，平均为 0.74）。只有一个样品的吸光度值低于所选截止值 0.4。 26 名健康护理人员的尿液样本中，除一份外，其余所有样本的 OD 值均低于 0.4 截止值。这些方法可能对于快速、简单地诊断结核病很有价值，特别是在缺乏生物安全 3 级 (BSL3) 设施的发展中国家。 (C) 2001 Elsevier Science B.V. 保留所有权利。
There is an urgent need for improved tools fbr laboratory diagnosis of active tuberculosis (TB), Here, we describe two methods, a catch-up ELISA and a dipstick test based on die detection in urine of lipoarabinomannan (LAM). LAM is a major and specific glycolipid component of the outer mycobacterial cell wall. Preliminary experiments showed that LAM is excreted in the urine of mice injected intraperitoneally with a crude cell wall preparation of Mycobacterium tuberculosis. Both methods were highly sensitive, detecting LAM at concentrations of 1 ng/ml and 5 pg/ml, respectively. Of 15 patients with active IB, all showed intermediate to high levels of LAM in their urine (absorbance values from 0.3 to 1.2, mean 0.74). Only one sample showed an absorbance value below the chosen cut off value of 0.4. All but one of the urine samples from 26 healthy nursing workers exhibited OD value below 0.4 cut off. These methods may prove valuable for rapid and simple diagnosis of TB in particular in developing countries lacking biosafety level 3 (BSL3) facilities. (C) 2001 Elsevier Science B.V. All rights reserved.