SPONTANEOUS DEATH OF ECHINOCOCCUS-MULTILOCULARIS - CASES DIAGNOSED SEROLOGICALLY (BY EM2 ELISA) AND CLINICAL-SIGNIFICANCE
SPONTANEOUS DEATH OF ECHINOCOCCUS-MULTILOCULARIS - CASES DIAGNOSED SEROLOGICALLY (BY EM2 ELISA) AND CLINICAL-SIGNIFICANCE
复制标题
DOI：
10.4269/ajtmh.1987.36.576
复制
发表时间：
1987-05-01
影响因子：
3.3
通讯作者：
MCMAHON, BJ
中科院分区：
文献类型：
--
作者：
RAUSCH, RL;WILSON, JF;MCMAHON, BJ
1985年，来自阿拉斯加州西部流行地区的爱斯基摩人中诊断出9例无症状肝脏泡球蚴病（AHD）病例。采用纯化的泡球蚴抗原（Em 2 ELISA），通过酶联免疫吸附试验（ELISA）筛选患者。五名病人，和一名诊断较早（1979年），被发现有病变，其中幼虫E。多房性在感染早期自发死亡。生存能力进行了组织学评估;通过抗生物素蛋白生物素免疫组织化学方法;并在体内通过腹膜内接种幼虫绦虫膜红背田鼠，Clethrionomys rutilus。根据计算机断层扫描和超声扫描的结果以及病变的肉眼外观，结果与临床印象一致，即绦虫死亡。E.人类多房性贫血以前没有报道过。结果表明，EM 2 ELISA可能是积极的患者有病变的AHD的病原体不再是可行的。
Nine cases of asymptomatic alveolar hydatid disease (AHD) of the liver were diagnosed in 1985 among Eskimos from the endemic region of western Alaska [USA]. The patients were identified by screening with the enzyme-linked immunosorbent assay, using purified Echinococcus multilocularis antigen (Em2 ELISA). Five patients, and one diagnosed earlier (1979), were found to have lesions in which the larval E. multilocularis had died spontaneously at an early stage of infection. Viability was assessed histologically; by the avidinbiotin immunohistochemical method; and in vivo through intraperitoneal inoculation of membranes of the larval cestode into red-backed voles, Clethrionomys rutilus. The results were in agreement with the clinical impression, based on findings by computerized tomography and ultrasound scanning, and on the macroscopic appearance of the lesions, that the cestode was dead. Spontaneous death of E. multilocularis in humans has not been previously reported. The findings show that the Em2 ELISA may be positive in patients having lesions of AHD in which the etiologic agent is no longer viable.