Iodidimonas gelatinilytica sp. nov., aerobic iodide-oxidizing bacteria isolated from brine water and surface seawater
Iodidimonas gelatinilytica sp. nov., aerobic iodide-oxidizing bacteria isolated from brine water and surface seawater
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DOI：
10.1007/s10482-021-01546-2
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发表时间：
2021-03-24
影响因子：
2.6
通讯作者：
Amachi, Seigo
中科院分区：
文献类型：
--
作者：
Iino, Takao;Oshima, Kenshiro;Amachi, Seigo
分别从日本千叶富碘天然气卤水和三重县表层海水中分离到化学有机营养型碘（I-）氧化细菌菌株Hi-2（T）和Mie-1。菌株Hi-2（T）和Mie-1的细胞是好氧的、革兰氏阴性的和杆状的（宽0.3-0.5 μ m，长1.2-4.4 μ m）。两个分离物在30 ° C、pH 7.5和3%NaCl（w/v）下生长最佳。碘氧化形成分子碘（I-2）是菌株Hi-2（T）和Mie-1的生物化学独特性状。主要的细胞脂肪酸是C-18：1 omega 7 c、C-16：1 omega 5c和C-18：1 2-OH。16 S rRNA基因序列分析表明，Hi-2（T）和Mie-1与穆里亚埃C-3（T）的同源性为99.2%。计算的两个分离物的DNA数字杂交（dDDH）值为65.7-65.9%，与I.穆里亚埃C-3（T）低于70%的阈值，该阈值用于原核生物种划分。菌株Hi-2（T）和Mie-1对秦皮甲素的水解、对明胶的水解以及对I.穆里亚埃C-3（T）。根据这些生化特性、主要的细胞脂肪酸组成和dDDH值，提出了菌株Hi-2（T）（= JCM 17844（T）= LMG 28661（T））和Mie-1（= JCM 17845 = LMG 28662）的一个新种，命名为Iodidimonas gelaginilytica。
Chemo-organotrophic iodide (I-)-oxidizing bacterial strains Hi-2(T) and Mie-1 were isolated from iodide-rich natural gas brine water in Chiba and surface seawater in Mie, Japan, respectively. Cells of strains Hi-2(T) and Mie-1 were aerobic, Gram-negative and rod-shaped (0.3-0.5 mu m width and 1.2-4.4 mu m in length). Two isolates grew optimally at 30 degrees C, pH 7.5 and with 3% NaCl (w/v). Iodide oxidation to form molecular iodine (I-2) was a biochemically unique trait for strains Hi-2(T) and Mie-1. The major cellular fatty acids are C-18:1 omega 7c, C-16:1 omega 5c and C-18:1 2-OH. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strains Hi-2(T) and Mie-1 were located near Iodidimonas muriae C-3(T) with 99.2% sequence similarity. The calculated digital DNA-DNA hybridization (dDDH) value of 65.7-65.9% between the two isolates and I. muriae C-3(T) was lower than the threshold of 70%, which was used for prokaryotic species delineation. Strains Hi-2(T) and Mie-1 differed in the hydrolysis of aesculin, the hydrolysis of gelatin and the major cellular fatty acids composition from I. muriae C-3(T). Considering these biochemical properties, the major cellular fatty acids composition and dDDH value, a novel species is proposed for strains Hi-2(T) (= JCM 17844(T) = LMG 28661(T)) and Mie-1 (= JCM 17845 = LMG 28662), to be named Iodidimonas gelatinilytica.