Screening assays for heart function mutants in Drosophila
Screening assays for heart function mutants in Drosophila
复制标题
DOI：
10.2144/04371st01
复制
发表时间：
2004-07-01
期刊：
影响因子：
2.7
通讯作者：
Bodmer, R
中科院分区：
文献类型：
--
作者：
Wessel, RJ;Bodmer, R
果蝇的快速生命周期和遗传可追溯性使其成为大规模遗传筛选的理想生物。在这里，我们描述了一种新的蛹心率和节律性测定方法，以及测量成人心脏应激反应的技术。这些检测可以有效地结合起来，同时筛选影响心脏功能的突变和影响成人心脏应激反应年龄依赖性下降的突变。在这种筛选中发现的突变有可能极大地有助于了解先天性心脏病和调节人口中年龄依赖性心功能下降。
The rapid life cycle and genetic tractability of Drosophila make it an ideal organism for large-scale genetic screens. Here we describe a novel assay for pupal heart rate and rhythmicity, as well as techniques to measure adult cardiac stress response. These assays can be powerfully combined to concurrently screen for both mutations affecting cardiac,function and mutations affecting the age-dependent decline in adult cardiac stress response. Mutations identified in such screens have the potential to contribute greatly to the understanding of both congenital heart disease and the regulation of age-dependent decline in cardiac function in the human population.