Pleiotropic changes revealed by in situ recovery of the semi-dwarf gene sd1 in rice
Pleiotropic changes revealed by in situ recovery of the semi-dwarf gene sd1 in rice
复制标题
水稻半矮化基因sd1的原位恢复揭示的多效性变化
DOI：
10.1016/j.jplph.2020.153141
复制
发表时间：
2020-05-01
影响因子：
4.3
通讯作者：
Traw, M. Brian
中科院分区：
文献类型：
--
作者：
Jia, Xianqing;Yu, Luyao;Traw, M. Brian
“绿色革命”在本世纪中期大幅降低了品种高度并大幅提高了水稻产量，这在很大程度上是通过半矮秆1（SD 1）缺陷等位基因的渐渗实现的，SD 1编码一种GA 20 ox氧化酶，参与水稻生物活性赤霉素合成的最后步骤。在这里，我们问是否转换有缺陷的sd 1版本在现代半矮秆品种回到野生型SD 1原位恢复祖先的植物性状，更广泛地说，它揭示了这个基因的多效性效应。我们评估了这些影响的SD 1恢复在三个独立的重组系回收的F-2后代的93-11和PA 64之间的交叉。然后，我们使用RNA-seq来剖析伴随SD 1恢复的基因网络变化。我们报告说，野生型SD 1的这种原位恢复几乎使株高增加一倍，增加每穗总产量，并延长第二片叶的长度。表达谱的比较揭示了赤霉素途径的关键节点，如OsKO 1和OsGA 2 ox 3，以及更广泛的代谢网络，防御反应和分解代谢过程相关基因的变化。两个JA诱导的基因，RIR 1b和OsPR 1b，非常下调后，SD 1恢复，这表明SD 1恢复改变了GA和JA之间的平衡，以降低植物的防御反应。在SD 1位点的原位方法也提供了一个适用于其他系统的模型实例，并将进一步了解作物高产性状的基因网络。
The "Green Revolution" that dramatically reduced cultivar heights and sharply boosted rice production mid-century was achieved in large part through introgression of defective alleles of Semi-Dwarf 1 (SD1), which encodes a GA20ox oxidase involved in the final steps of the synthesis of bioactive gibberellin in rice. Here, we ask whether converting the defective sd1 version in a modern semi-dwarf cultivar back to wild-type SD1 in situ recovers ancestral plant traits, and more broadly, what it reveals about pleiotropic effects of this gene. We assess these effects of SD1 restoration in three independent recombinant lines recovered from F-2 progeny of a cross between 93-11 and PA64s. We then used RNA-seq to dissect gene network changes that accompanied SD1 restoration. We report that this in situ restoration of wild-type SD1 nearly doubles plant height, increases total grain yield per panicle, and elongates the second-leaf length. Comparison of expression profiles reveals changes in key nodes of the gibberellin pathway, such as OsKO1 and OsGA2ox3, and more broadly in genes related to metabolic networks, defense response, and catabolic processes. Two JA-induced genes, RIR1b and OsPR1b, are extremely down-regulated after SD1 restoration, suggesting that SD1 restoration alters the balance between GA and JA to plant growth, at the cost of degrading the defense response. This in situ approach at the SD1 locus also provides a model example that is applicable to other systems and will further understanding of gene networks underlying high-yield traits in crops.