Traditional preparations of kava (Piper methysticum) inhibit the growth of human colon cancer cells in vitro
Traditional preparations of kava (Piper methysticum) inhibit the growth of human colon cancer cells in vitro
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DOI：
10.1016/j.phymed.2016.11.002
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发表时间：
2017-01-15
期刊：
影响因子：
7.9
通讯作者：
Balick, M. J.
中科院分区：
文献类型：
--
作者：
Einbond, L. S.;Negrin, A.;Balick, M. J.
背景资料：流行病学研究表明，在南太平洋岛屿，包括斐济、西萨摩亚和瓦努阿图，结肠癌的发病率很低。癌症发病率已被证明与卡瓦（Piper methysticum G. Forst.）假设/目的：传统制备的卡瓦将抑制人类癌细胞的生长。这项调查需要制备和分析卡瓦提取物和研究的生长抑制活性的提取物，单独和组合与hibiscus.Study设计：我们将准备卡瓦作为在密克罗尼西亚-作为水提取物，颗粒含量高，单独或组合与海芙蓉（Hibiscus tiliaceus L.）- 方法：我们从密克罗尼西亚不同来源的侧根和海葵粘液和树液中获得以传统方式制备的研磨卡瓦，并制备水提取物（未过滤的，以及过滤的，因为在传统使用中卡瓦饮料含有高颗粒含量）和分区。采用MTT法测定了该制剂对结肠癌细胞、乳腺癌细胞和非恶性肠上皮细胞的生长抑制活性。LC-MS分析用于检查卡瓦和海葵提取物和partitions.Results的组成部分：卡瓦的传统制剂抑制乳腺癌和结肠癌细胞的生长。在卡瓦制剂中，活性降低的顺序为斐济（2）、斐济（1）、夏威夷;来自斐济的未过滤制剂比过滤制剂活性更高。植物化学分析表明，过滤减少了大部分卡法根内酯和查耳酮的含量。例如，对于Fiji（2），过滤/未过滤卡法根中二氢麻醉椒苦素的比例为0.01。因此，对于来自斐济的提取物，生长抑制活性与这些化合物的含量相关。来自斐济的未过滤和过滤卡瓦（1）对恶性肠上皮细胞的活性高于非恶性肠上皮细胞。由于卡法根是在密克罗尼西亚通过将提取物挤压通过海葵树皮来制备的，我们测定了卡法根和海葵汁液的组合的生长抑制活性，发现海葵增强了卡法根的生长抑制作用。我们的研究结果表明，传统的卡瓦，单独或与海芙蓉，显示出对人类癌细胞的活性，并表明值得开发和进一步分析这些制剂以预防和治疗结肠癌和其他癌症。我们的研究结果表明，重要的是要检查植物在人们消费时的活动。(C)2016 Elsevier GmbH. All rights reserved.
Background: Epidemiological studies indicate there is low incidence of colon cancer in the South Pacific islands, including Fiji, West Samoa, and Vanuatu. Cancer incidence has been shown to be inversely associated with kava (Piper methysticum G. Forst.) ingestion.Hypothesis/Purpose: Kava prepared traditionally will inhibit the growth of human cancer cells. This investigation entails preparation and analysis of kava extracts and study of the growth inhibitory activity of the extracts, alone and combined with hibiscus.Study Design: We will prepare kava as in Micronesia - as a water extract, high in particulate content, alone or combined with sea hibiscus (Hibiscus tiliaceus L.) - and examine the components and growth inhibitory activity.Methods: We obtained ground kava prepared in the traditional way from lateral roots and sea hibiscus mucilage and sap from different sources in Micronesia, and prepared water extracts (unfiltered, as well as filtered, since in traditional use the kava beverage contains a high particulate content) and partitions. We used the MTT assay to determine the growth inhibitory activity of the preparations on colon and breast cancer cells and nonmalignant intestinal epithelial cells. LC-MS analysis was used to examine the components of the kava and sea hibiscus extracts and partitions.Results: Traditional preparations of kava inhibit the growth of breast and colon cancer cells. Among the kava preparations, the order of decreasing activity was Fiji(2), Fiji(1), Hawaii; the unfiltered preparations from Fiji were more active than the filtered. Phytochemical analysis indicated that filtering reduced most kavalactone and chalcone content. For example, for Fiji(2), the ratio of dihydromethysticin in filtered/ unfiltered kava was 0.01. Thus, for the extracts from Fiji, growth inhibitory activity correlates with the content of these compounds. Unfiltered and filtered kava from Fiji(1) were more active on malignant than nonmalignant intestinal epithelial cells. Since kava is prepared in Micronesia by squeezing the extract through sea hibiscus bark, we assayed the growth inhibitory activity of combinations of kava and sea hibiscus sap and found that sea hibiscus enhanced the growth inhibitory effect of kava.Conclusion: Our results show that traditional kava, alone or combined with sea hibiscus, displays activity against human cancer cells and indicate it will be worthwhile to develop and further analyze these preparations to prevent and treat colon and other cancers. Our findings suggest it is important to examine the activity of plants in the form that people consume them. (C) 2016 Elsevier GmbH. All rights reserved.