Pti1p and Ref2p found in association with the mRNA 3′ end formation complex direct snoRNA maturation
Pti1p and Ref2p found in association with the mRNA 3′ end formation complex direct snoRNA maturation
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DOI：
10.1093/emboj/cdg253
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发表时间：
2003-06-02
期刊：
影响因子：
11.4
通讯作者：
Minvielle-Sebastia, L
中科院分区：
文献类型：
--
作者：
Dheur, S;Vo, LTA;Minvielle-Sebastia, L
真核RNA聚合酶II转录mRNAs和非蛋白质编码的RNAs的前体，如SnRNAs和snoRNAs。这些RNA必须在其3‘端进行处理才能发挥作用。MRNAs是通过切割和多聚腺苷作用成熟的，这需要一个具有良好特性的蛋白质复合体。小的RNA也会受到3‘端的切割，但不是多腺化的。在这里，我们证明了两个新发现的蛋白质，Pti1p和Ref2p，虽然它们被发现与前mRNA 3‘端加工复合体相关，但对酵母snoRNA3’端成熟是必不可少的。我们还提供了证据，证明Pti1p可能通过解偶联的切割和多聚腺苷作用，并与Nrd1p依赖的途径协同作用，形成非多腺化转录本的3‘端。
Eukaryotic RNA polymerase II transcribes precursors of mRNAs and of non-protein-coding RNAs such as snRNAs and snoRNAs. These RNAs have to be processed at their 3' ends to be functional. mRNAs are matured by cleavage and polyadenylation that require a well-characterized protein complex. Small RNAs are also subject to 3' end cleavage but are not polyadenylated. Here we show that two newly identified proteins, Pti1p and Ref2p, although they were found associated with the pre-mRNA 3' end processing complex, are essential for yeast snoRNA 3' end maturation. We also provide evidence that Pti1p probably acts by uncoupling cleavage and polyadenylation, and functions in coordination with the Nrd1p-dependent pathway for 3' end formation of non-polyadenylated transcripts.