Development of Imaging Mass Spectrometry
Development of Imaging Mass Spectrometry
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DOI：
10.1248/bpb.b212007
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发表时间：
2012-09-01
影响因子：
2
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Saito, Yusuke;Waki, Michihiko;Setou, Mitsutoshi
我们已经开发了一种质量显微镜，其中显微镜与高分辨率基质辅助激光解吸/电离成像质谱（MALDI-IMS）相结合。该技术是一种强大的工具，用于研究生物分子的空间分布，而不需要任何耗时的提取，纯化和标记生物组织切片的程序。质量显微镜在一次测量中提供关于数百个生物分子分布的清晰图像，并且还有助于确定生物系统的细胞概况。在这篇综述中，我们集中在临床应用中的一些最新进展，并描述了如何质量显微镜可以用来评估病理形态学和药代动力学。
We have developed a mass microscope in which a microscope is combined with high-resolution matrix assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). This technique is a powerful tool for investigating the spatial distribution of biomolecules without the need for any time-consuming extraction, purification, and labeling procedures for biological tissue sections. The mass microscope provides clear images with regards to the distribution of hundreds of biomolecules in a single measurement, and also helps in determining the cellular profile of the biological system. In this review, we focus on some of the recent developments in clinical applications and describe how the mass microscope can be employed to assess pathomorphology and pharmacokinetics.