The effect of the intestinal flora on the growth rate of mice, and on their susceptibility to experimental infections.
The effect of the intestinal flora on the growth rate of mice, and on their susceptibility to experimental infections.
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DOI：
10.1084/jem.111.3.407
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发表时间：
1960-03-01
期刊：
影响因子：
--
通讯作者：
SCHAEDLER RW
中科院分区：
文献类型：
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作者：
DUBOS RJ;SCHAEDLER RW
剖腹产的小鼠被用来发展一个新的小鼠群体，该群体一直保持在一个不与常见小鼠病原体接触的环境中，但不处于无菌状态。这些小鼠被命名为NCS，并与来自正常条件下维持的亲代群体的相同性别和年龄的动物进行比较。在完全饮食的情况下，NCS小鼠的生长速度比普通小鼠更快；此外，当喂食导致普通小鼠停止生长或体重减轻的不足饮食时，它们的体重继续增加-尽管增加的速度较慢。事实证明，NCS小鼠比普通小鼠更容易受到某些实验性细菌感染。相比之下，它们比普通老鼠对大剂量内毒素的致死作用更具抵抗力。然而，注射微量这些内毒素后，他们对葡萄球菌感染的易感性显著增加。细菌学研究表明，NCS小鼠与普通小鼠的肠道菌群存在明显的质的差异。当NCS小鼠被从普通小鼠的肠道中回收的一种大肠杆菌污染时，它们获得了后一种动物在各种饮食下的体重增加以及对细菌病原体和内毒素的反应的特征。NCS小鼠已被发现非常适合于研究几个营养、细菌学和免疫学问题，似乎它们的大规模生产不会构成不可逾越的困难。
Mice delivered by Caesarian section were used to develop a new mouse colony which has been maintained in an environment protected from contact with common mouse pathogens, but not in the germ-free state. These mice, designated as NCS, were compared with animals of the same sex and age coming from the parent colony maintained under ordinary conditions. The NCS mice grew more rapidly than ordinary mice on complete diets; moreover, they continued to gain weight—although at a slower rate—when fed deficient diets which caused ordinary mice to stop growing, or to lose weight. The NCS mice proved much more susceptible than ordinary mice to certain experimental bacterial infections. In contrast, they were much more resistant than ordinary mice to the lethal effect of large doses of endotoxins. However, they responded to injection of minute amounts of these endotoxins by a marked increase in susceptibility to staphylococcal infection. Bacteriological studies revealed striking qualitative differences between the intestinal flora of NCS and ordinary mice. When NCS mice were contaminated—either by contact or by feeding—with a strain of Escherichia coli recovered from the intestine of ordinary mice, they acquired the characteristics of the latter animals with regard to weight gain on various diets, and to response to bacterial pathogens and endotoxins. NCS mice have been found well suited to the study of several nutritional, bacteriological, and immunological problems and it appears that their production on a large scale will not present unsurmountable difficulties.