Gaining or cutting SLAC: the evolution of plant guard cell signalling pathways
Gaining or cutting SLAC: the evolution of plant guard cell signalling pathways
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DOI：
10.1101/2021.05.26.445736
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发表时间：
2021-05
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通讯作者：
F. Sussmilch;T. Maierhofer;Johannes Herrmann;Lena J. Voss;Christof Lind;Maxim Messerer;Heike M. Müller;Maria S. Bünner;P. Ache;Klaus F. X. Mayer;D. Becker;M. Roelfsema;D. Geiger;J. Schultz;R. Hedrich
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作者：
F. Sussmilch;T. Maierhofer;Johannes Herrmann;Lena J. Voss;Christof Lind;Maxim Messerer;Heike M. Müller;Maria S. Bünner;P. Ache;Klaus F. X. Mayer;D. Becker;M. Roelfsema;D. Geiger;J. Schultz;R. Hedrich
可调节植物气孔的进化，使角质层蜡封组织中的CO2获取成为陆地生命发展中最重要的事件之一。但是，调节气孔运动的保卫细胞信号通路是如何进化的呢？我们通过比较蕨类植物和被子植物保卫细胞转录组来研究这一点。我们发现，这些不同的植物群体在保卫细胞中共享相似基因的表达，包括干旱胁迫激素脱落酸（阿坝）的生物合成和信号基因。然而，尽管在保卫细胞中保守表达，但SLAC/SLAH家族的S型阴离子通道-已知在被子植物中用于ABA介导的气孔关闭-在蕨类植物中不被相同的途径激活，突出了功能上的可能差异。其他陆地植物通道的研究揭示了一个复杂的进化历史，具有多个增益或损失的SLAC激活机制，因为这些通道被招募到气孔关闭的作用。总之，开花和非开花植物的保卫细胞具有相似的核心特征，但也表现出谱系特异性和生态位相关的适应性，可能是行为差异的基础。
The evolution of adjustable plant pores (stomata), enabling CO2 acquisition in cuticle wax-sealed tissues was one of the most significant events in the development of life on land. But how did the guard cell signalling pathways that regulate stomatal movements evolve? We investigate this through comparison of fern and angiosperm guard cell transcriptomes. We find that these divergent plant groups share expression of similar genes in guard cells including biosynthesis and signalling genes for the drought stress hormone abscisic acid (ABA). However, despite conserved expression in guard cells, S-type anion channels from the SLAC/SLAH family – known for ABA-mediated stomatal closure in angiosperms – are not activated by the same pathways in ferns, highlighting likely differences in functionality. Examination of other land plant channels revealed a complex evolutionary history, featuring multiple gains or losses of SLAC activation mechanisms, as these channels were recruited to a role in stomatal closure. Taken together, the guard cells of flowering and non-flowering plants share similar core features, but also show lineage-specific and ecological niche-related adaptations, likely underlying differences in behaviour.