Sequential Drug Release and Enhanced Photothermal and Photoacoustic Effect of Hybrid Reduced Graphene Oxide-Loaded Ultrasmall Gold Nanorod Vesicles for Cancer Therapy.
Sequential Drug Release and Enhanced Photothermal and Photoacoustic Effect of Hybrid Reduced Graphene Oxide-Loaded Ultrasmall Gold Nanorod Vesicles for Cancer Therapy.
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DOI：
10.1021/acsnano.5b03804
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发表时间：
2015-09-22
期刊：
影响因子：
17.1
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Song J;Yang X;Jacobson O;Lin L;Huang P;Niu G;Ma Q;Chen X
我们报道了一种混合还原氧化石墨烯（rGO）负载的超小等离子体金纳米棒囊泡（rGO-AuNRVe）（尺寸约65 nm），具有显著的放大光声（PA）性能和光热效应。该混合囊泡还表现出高的阿霉素（DOX）负载能力，因为囊泡的腔和包封的rGO的大表面积都可以用于负载DOX，使其成为优异的药物载体。负载的DOX依次释放：近红外光热加热诱导DOX从囊泡腔释放，细胞内酸性环境诱导DOX从rGO表面释放。正电子发射断层扫描成像显示64 Cu标记的rGO-AuNRVes在U87 MG肿瘤中的高被动积累（注射后24 h约为9.7% ID/g）和肿瘤区域中的强PA信号。单次静脉注射rGO-AuNRVe-DOX，然后低功率密度808 nm激光照射（0.25 W/cm 2）显示，由于化疗和光热疗法的组合，有效抑制肿瘤生长。能够通过激光和酸性环境顺序释放DOX的rGO-AuNRVe-DOX可能具有临床转化的潜力，以治疗具有光可接近的肿瘤的癌症患者。
We report a hybrid reduced graphene oxide (rGO)-loaded ultrasmall plasmonic gold nanorod vesicle (rGO-AuNRVe) (~65 nm in size) with remarkably amplified photoacoustic (PA) performance and photothermal effects. The hybrid vesicle also exhibits a high loading capacity of doxorubicin (DOX), as both the cavity of the vesicle and the large surface area of the encapsulated rGO can be used for loading DOX, making it an excellent drug carrier. The loaded DOX is released sequentially: near-infrared photothermal heating induces DOX release from the vesicular cavity, and an intracellular acidic environment induces DOX release from the rGO surface. Positron emission tomography imaging showed high passive U87MG tumor accumulation of 64Cu-labeled rGO-AuNRVes (~9.7% ID/g at 24 h postinjection) and strong PA signal in the tumor region. Single intravenous injection of rGO-AuNRVe-DOX followed by low-power-density 808 nm laser irradiation (0.25 W/cm2) revealed effective inhibition of tumor growth due to the combination of chemo- and photothermal therapies. The rGO-AuNRVe-DOX capable of sequential DOX release by laser light and acid environment may have the potential for clinical translation to treat cancer patients with tumors accessible by light.