Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells
Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells
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DOI：
10.1016/j.neulet.2005.11.057
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发表时间：
2006-04-10
影响因子：
2.5
通讯作者：
Nomura, Y
中科院分区：
文献类型：
--
作者：
Hayashi, N;Shirakura, H;Nomura, Y
本研究的目的是阐明SUMO-1修饰在caspase-7在神经细胞中的作用。我们以前已经证明，procaspase-2可能是SUMO-1修饰的可能靶点。在本研究中，我们试图调查是否其他半胱天冬酶也与Ubc 9/SUMO-1相互作用。在哺乳动物细胞中观察到SUMO-1与caspase-7的特异性结合。缺失突变分析显示SUMO-1修饰位点至少位于caspase-7的N端p20亚基。此外，SUMO-1修饰的caspase-7在核定位中呈现点状结构。这些研究结果表明，SUMO-1修饰caspase-7可能与其在细胞核中的特异性定位有关，因此可能有助于神经元凋亡过程中核底物的裂解。(C)2005爱思唯尔爱尔兰有限公司保留所有权利。
The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis. (C) 2005 Elsevier Ireland Ltd. All rights reserved.