Schistosoma mansoni and Echinostoma caproni excretory-secretory products differentially affect gene expression in Biomphalaria glabrata embryonic cells
Schistosoma mansoni and Echinostoma caproni excretory-secretory products differentially affect gene expression in Biomphalaria glabrata embryonic cells
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DOI：
10.1017/s0031182003004049
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发表时间：
2003-12-01
期刊：
影响因子：
2.4
通讯作者：
Modat, S
中科院分区：
文献类型：
--
作者：
Coustau, C;Mitta, G;Modat, S
光滑双脐胚胎 (Bge) 细胞已被证明是研究蜗牛宿主与寄生虫相互作用的有价值的体外细胞模型。它们都促进各种吸虫物种的生长和分化，包括曼氏血吸虫和卡普罗尼棘口吸虫，并与循环血细胞共享一些形态和功能特征。作为研究暴露于吸虫排泄物 (ES) 产物后可能受到调节的蜗牛基因的一种方法，我们比较了暴露于盐水溶液或含有来自 S. mansoni 或 E. caproni（寄生于光滑 B. glabrata 的两种吸虫）的 ES 产品的盐水溶液的 Bge 细胞的基因表达谱。在差异显示 RT-PCR 分析之后，我们对 23 个差异显示的 cDNA 进行了表征，并重点关注与已知基因显示序列相似性的 5 个 cDNA，以进行表达验证。使用 RT-PCR，我们证实来自 S. mansoni 和 E. caproni 的 ES 产物对 5 个转录物中的 4 个转录物的表达水平有差异性影响。这些部分转录物对应于新的光滑芽孢杆菌序列，并且与编码来自 Kunitz 家族的 (i) 细胞色素 C、(ii) 甲基结合蛋白、(iii) 谷氨酰胺合成酶和 (iv) 蛋白酶抑制剂的基因显示出显着的序列相似性。讨论了这些基因表达变化在宿主-寄生虫分子相互作用中的可能意义。
Biomphalaria glabrata embryonic (Bge) cells have been shown to be a valuable in vitro cellular model for the study of snail host-parasite interactions. They both promote the growth and differentiation of various trematode species including Schistosoma mansoni, and Echinostoma caproni and share some morphological and functional features with circulating haemocytes. As an approach to investigate snail genes potentially regulated following exposure to trematode excretory secretory (ES) products, we compared gene expression profiles of Bge cells exposed to saline solution, or saline solution containing ES products from S. mansoni or E. caproni, two trematode species parasitizing B. glabrata. Following differential display RT-PCR analysis we characterized 23 differentially displayed cDNAs and we focussed on the 5 cDNAs showing sequence similarity to known genes for expression validation. Using RT-PCR, we confirmed that ES products from S. mansoni and E. caproni differentially affect the expression levels of 4 out of the 5 transcripts. These partial transcripts corresponded to novel B. glabrata sequences, and showed significant sequence similarity to genes coding for (i) cytochrome C, (ii) methyl-binding proteins, (iii) glutamine synthetases, and (iv) protease inhibitors from the Kunitz family. The possible significance of these gene expression changes in host-parasite molecular interactions is discussed.