Localized neurite outgrowth sensing via substrates with alternative rigidities
Localized neurite outgrowth sensing via substrates with alternative rigidities
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DOI：
10.1039/c1sm06336g
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发表时间：
2011-01-01
期刊：
影响因子：
3.4
通讯作者：
LeDuc, Philip R.
中科院分区：
文献类型：
--
作者：
Chou, Szu-Yuan;Cheng, Chao-Min;LeDuc, Philip R.
本文描述了局部基底刚度对神经反应的影响（例如，例如，在一个实施例中，神经突生长及其路径）。为了解决这个问题，我们创造了一种方法来控制细胞外基质的微环境的顺应性，通过生成一个聚合物基质与交替的弹性，与神经元相互作用。我们制作的聚（二甲基硅氧烷）通道组成的局部表面的弹性模量为800 kPa和200 kPa相邻。然后，我们接种和分化的神经母细胞，并发现他们的过程中表现出不同的模式，因为他们接近和交叉的弹性定义的多柔度聚合物复合材料的接口。根据索马的位置和与弹性界面相比时的生长方向，生长将以不同的路径向前延伸，包括折回、沿沿着对齐或穿过弹性界面边界。这些结果表明，在分化成神经细胞的过程中，局部基底弹性对神经突生长有深远的影响。
This paper describes the influence of localized substrate stiffness on neural responses (e. g., neurite outgrowth and its path) in neuroblasts. To address this issue, we created a method to control the microenvironment of extracellular matrix compliances by generating a polymer matrix with alternating elasticities that interacted with neurons. We fabricated poly(dimethylsiloxane) channels comprised of localized surfaces with elastic moduli of 800 kPa and 200 kPa adjacent to one another. We then seeded and differentiated neuroblasts, and found that their processes exhibited distinct patterns as they approached and crossed the elastically defined interfaces of the multi-compliance polymer composite. Depending on the location of the soma and the direction of the outgrowth when compared to the elasticity interface, the outgrowths would extend forward in distinct paths including turning back, aligning along, or crossing the elasticity interface boundary. These results indicate that localized substrate elasticity has profound effects on neurite outgrowth in differentiating neuroblasts.