Involvement of matrix metalloproteinase in neuroblast cell migration from the subventricular zone after stroke
Involvement of matrix metalloproteinase in neuroblast cell migration from the subventricular zone after stroke
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DOI：
10.1523/jneurosci.4085-05.2006
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发表时间：
2006-03-29
影响因子：
5.3
通讯作者：
Lo, EH
中科院分区：
文献类型：
--
作者：
Lee, SR;Kim, HY;Lo, EH
脑损伤后，来自脑室下区（SVZ）的成神经细胞扩张并向受损组织迁移。介导这些神经原性和迁移反应的机制仍有待充分解剖。在这里，我们表明，在小鼠短暂局灶性脑缺血后2周的恢复期内，来自SVZ的溴脱氧尿苷标记和双皮质素阳性细胞与细胞外蛋白酶基质金属蛋白酶-9（MMP-9）共定位。用广谱MMP抑制剂GM 6001治疗显著降低了从SVZ延伸到纹状体的双皮质素阳性细胞的迁移。这些数据表明，基质金属蛋白酶参与内源性机制的神经原性迁移的大脑寻求愈合后受伤。
After brain injury, neuroblast cells from the subventricular zone ( SVZ) expand and migrate toward damaged tissue. The mechanisms that mediate these neurogenic and migratory responses remain to be fully dissected. Here, we show that bromodeoxyuridine-labeled and doublecortin-positive cells from the SVZ colocalize with the extracellular protease matrix metalloproteinase-9 (MMP-9) during the 2 week recovery period after transient focal cerebral ischemia in mice. Treatment with the broad spectrum MMP inhibitor GM6001 significantly decreases the migration of doublecortin-positive cells that extend from the SVZ into the striatum. These data suggest that MMPs are involved in endogenous mechanisms of neurogenic migration as the brain seeks to heal itself after injury.