AN1/A20 zinc-fi nger proteins positively regulate major latex-likeproteins, transporting factors for dioxin-like compounds inCucurbita pepo
AN1/A20 zinc-fi nger proteins positively regulate major latex-likeproteins, transporting factors for dioxin-like compounds inCucurbita pepo
复制标题
AN1/A20 锌指蛋白正向调节葫芦中二恶英类化合物的主要乳胶样蛋白和转运因子
DOI：
10.1016/j.chemosphere.2022.135536
复制
发表时间：
2022
期刊：
影响因子：
8.8
通讯作者：
Inui H
中科院分区：
文献类型：
--
作者：
Fujita K;Yoshihara R;Hirota M;Goto J;Sonoda C;Inui H
葫芦科植物在果实中积累类似二恶英的化合物。我们先前的研究表明，A20/AN1锌指蛋白(ZFP)基因在西葫芦(Cucurbita Pepo)亚种中高度表达，这种亚种在高浓度下积累二恶英类化合物。过量表达A20/AN1 ZFP基因的转基因烟草植株在其上部积累了类似二恶英的化合物。然而，受A20/AN1 ZFP调控的二恶英类化合物积累的机制仍不清楚。在这里，我们证明了A20/AN1 ZFP正向调节主要的乳胶样蛋白(MLP)及其同源基因Inn的表达。烟草和C.佩波。MLP参与了二恶英类化合物从根部到上部的运输。佩波。A20/AN1 ZFP基因的过表达。与二恶英类化合物结合能力强的致病相关蛋白10类基因表达上调。我们的结果表明，A20/AN1 ZFP上调了MLP及其同源基因Inn。烟草和C.PEPO，导致二恶英类化合物的积累。
The Cucurbitaceae family accumulates dioxin-like compounds in its fruits. We previously showed thatA20/AN1zinc finger protein(ZFP) genes were highly expressed in the zucchini (Cucurbita pepo) subspeciespepo, which accumulates dioxin-like compounds at high concentrations. Transgenic tobacco (Nicotiana tabacum) plants overexpressingA20/AN1 ZFPgenes show accumulation of dioxin-like compounds in their upper parts. However, the mechanisms underlying the accumulation of dioxin-like compounds regulated by the A20/AN1 ZFPs remain unclear. Here, we show that A20/AN1 ZFPs positively regulate the expression of themajor latex-like protein(MLP) and its homolog genes inN. tabacumandC. pepo. MLPs are involved in the transport of dioxin-like compounds from the roots to the upper parts ofC. pepo. Overexpression ofA20/AN1 ZFPgenes inN. tabacumleads to the upregulation ofpathogenesis-related protein class-10genes with the binding ability toward dioxin-like compounds. Our results demonstrated that A20/AN1 ZFPs upregulateMLPand its homolog genes inN. tabacumandC. pepo, resulting in the accumulation of dioxin-like compounds.