Testicular Synthesis and Vitamin D Action
Testicular Synthesis and Vitamin D Action
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DOI：
10.1210/jc.2014-1690
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发表时间：
2014-10-01
影响因子：
5.8
通讯作者：
Obermayer-Pietsch, B.
中科院分区：
文献类型：
--
作者：
Hofer, D.;Muenzker, J.;Obermayer-Pietsch, B.
背景：维生素 D 系统不仅在骨代谢中具有多效作用。它在睾丸类固醇生成中的作用是新的，值得深入研究。目的：我们假设维生素 D，尤其是 1,25 二羟基维生素 D-3 [1,25(OH)(2)D-3（骨化三醇）] 诱导男性类固醇生成，并打算确定其对睾丸雄激素调节中的基因和途径的影响。方法：人成人原代睾丸细胞 分离并用 1,25(OH)(2)D-3 处理，并通过微阵列分析分析其基因表达水平。通过实时定量PCR证实了高度调控的基因。此外，还评估了1,25(OH)(2)D-3与LH和IGF-I组合对雄激素基因表达水平的影响。培养基中的 T 水平通过高分辨率 ELISA 测定。使用免疫细胞化学在基线和 1,25(OH)(2)D-3 刺激后确认了维生素 D 受体的表达。结果：微阵列描绘了 63 个受 1,25(OH)(2)D-3 显着调节的基因，包括与男性雄激素和维生素 D 代谢相关的基因，主要由维生素 D 受体/类视黄醇 X 受体激活触发。 1,25(OH) 2D3 导致人类睾丸细胞培养物中生殖基因表达谱的显着变化，并显着增加 T 合成。结论：来自我们的人类原代睾丸细胞培养模型的数据表明，维生素 D 在体外男性类固醇生成中发挥着重要作用。
Context: The vitamin D system has pleiotropic effects not only in bone metabolism. Its role in testicular steroidogenesis is new and deserves intensive research.Objective: We hypothesize that vitamin D, especially 1,25 dihydroxyvitamin D-3 [1,25(OH)(2)D-3 (calcitriol)] induces male steroidogenesis and intend to identify its impact on genes and pathways in testicular androgen regulation.Methods: Human adult primary testicular cells were isolated, treated with 1,25(OH)(2)D-3, and their gene expression levels profiled by microarray analysis. Highly regulated genes were confirmed by real-time quantitative PCR. In addition, the effects of 1,25(OH)(2)D-3 in combination with LH and IGF-I on the gene expression level of androgens were assessed. T levels in the culture media were determined by a high-resolution ELISA. The expression of vitamin D receptor was confirmed at baseline and after 1,25(OH)(2)D-3 stimulation using immunocytochemistry.Results: Microarrays depicted 63 genes significantly regulated by 1,25(OH)(2)D-3, including genes related to male androgen and vitamin D metabolism, mainly triggered by the vitamin D receptor/retinoid X receptor activation. 1,25(OH) 2D3 led to significant changes in the expression profiles of reproductive genes and significantly increased T synthesis in human testicular cell cultures.Conclusions: Data from our human primary testicular cell culture model suggest that vitamin D plays a major role in male steroidogenesis in vitro.