Enhanced phytoremediation of volatile environmental pollutants with transgenic trees
Enhanced phytoremediation of volatile environmental pollutants with transgenic trees
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DOI：
10.1073/pnas.0703276104
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发表时间：
2007-10-23
影响因子：
11.1
通讯作者：
Strand, Stuart E.
中科院分区：
文献类型：
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作者：
Doty, Sharon L.;James, C. Andrew;Strand, Stuart E.
小分子挥发性碳氢化合物，包括三氯乙烯、氯乙烯、四氯化碳、苯和氯仿，是常见的环境污染物，对健康造成严重影响。我们开发了转基因杨树（Populus tremula x Populus alba）植物，通过细胞色素 P450 2E1（多种卤代化合物代谢中的关键酶）的过度表达，大大提高了代谢率和去除这些污染物的速度。转基因杨树植物对水培溶液中这些污染物的去除率有所提高。当植物暴露于气态三氯乙烯、氯仿和苯时，它们也表现出优异的去除空气中污染物的能力。鉴于它们的大尺寸和广泛的根系，这些转基因杨树可以提供有效修复被各种污染物污染的场地的方法，其速度比目前的传统技术更快，成本更低。
Small, volatile hydrocarbons, including trichloroethylene, vinyl chloride, carbon tetrachloride, benzene, and chloroform, are common environmental pollutants that pose serious health effects. We have developed transgenic poplar (Populus tremula x Populus alba) plants with greatly increased rates of metabolism and removal of these pollutants through the overexpression of cytochrome P450 2E1, a key enzyme in the metabolism of a variety of halogenated compounds. The transgenic poplar plants exhibited increased removal rates of these pollutants from hydroponic solution. When the plants were exposed to gaseous trichloroethylene, chloroform, and benzene, they also demonstrated superior removal of the pollutants from the air. In view of their large size and extensive root systems, these transgenic poplars may provide the means to effectively remediate sites contaminated with a variety of pollutants at much faster rates and at lower costs than can be achieved with current conventional techniques.