In vitro regulation of local secretion and contraction of the bovine oviduct: stimulation by luteinizing hormone, endothelin-1 and prostaglandins, and inhibition by oxytocin.
In vitro regulation of local secretion and contraction of the bovine oviduct: stimulation by luteinizing hormone, endothelin-1 and prostaglandins, and inhibition by oxytocin.
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牛输卵管局部分泌和收缩的体外调节：黄体生成素、内皮素-1和前列腺素的刺激，以及催产素的抑制。
作者：
M. Wijayagunawardane;Akio Miyamoto;Y. Taquahashi;Christoph Gabler;Tomas J. Acosta;M. Nishimura;Gary J. Killian;K. Sato
牛周期性输卵管收缩的精确调节机制尚不清楚。本研究的目的是评估黄体生成素（LH）、类固醇、前列腺素（PG）和肽对输卵管收缩以及PG和内皮素（ET-1）分泌的影响。此外，通过逆转录聚合酶链反应（RT-PCR）评估了 ET-1 及其受体（ET-R）的 mRNA 循环表达。在体外微透析研究中，单独输注 LH 或与黄体酮 (P(4))、雌二醇-17β (E(2)) 和/或 ET-1 联合输注可刺激卵泡期和排卵期奶牛输卵管中 PGE(2)、PGF(2α) 和 ET-1 的显着释放。向培养基中添加LH、LH+P(4)+E(2)和/或ET-1增加了输卵管收缩的幅度。然而，催产素（OT）完全阻断了输卵管分泌和收缩的反应。相比之下，这些物质对黄体中期奶牛的输卵管没有显示出任何影响。编码ET-R A型和B型的mRNA表达模式相似，在排卵后阶段表达最高，在黄体期表达较低，在卵泡期表达最低。我们认为，排卵前 LH 激增，加上 Graafian 卵泡 E(2) 水平的增加和黄体 (CL) 退化的基础 P(4) 水平，刺激输卵管最大程度地产生 PG 和 ET-1，导致输卵管收缩，以实现配子的快速运输。新形成的 CL 释放的 OT 可能会阻断这些机制，并减缓胚胎运输到子宫的收缩。
The precise regulatory mechanisms of cyclic oviductal contraction in the cow are unclear. The purpose of this study was to evaluate the effect of luteinizing hormone (LH), steroids, prostaglandins (PGs) and peptides on the oviductal contraction and secretion of PGs and endothelin (ET-1). In addition, the cyclic expression of mRNA for ET-1 and its receptors (ET-R) was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). In the in vitro microdialysis study, an infusion of LH alone or in combination with progesterone (P(4)), estradiol-17beta (E(2)) and/or ET-1 stimulated pronounced release of PGE(2), PGF(2alpha) and ET-1 in the oviducts from cows in the follicular and postovulatory phases. The addition of LH, LH+P(4)+E(2) and/or ET-1 to the medium increased the amplitude of oviductal contraction. However, oxytocin (OT) completely blocked the responses of oviductal secretion and contraction. In contrast, these substances did not show any effect in the oviducts from cows in the mid luteal phase. Similar expression patterns of mRNA encoding for ET-R type A and type B were found, which were highest during the postovulatory phase, lower during the luteal phase, with the lowest expression during the follicular phase. We suggest that the preovulatory LH surge, together with increasing E(2) levels from the Graafian follicle and a basal P(4) from regressing corpora lutea (CL), stimulates maximum oviductal production of PG and ET-1, resulting in oviductal contraction for a rapid transport of gametes. OT released from the newly-formed CL may block these mechanisms, and slow contractions for transport of the embryo to the uterus.
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DOI：
10.1210/endo.137.7.8770916
发表时间：
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期刊：
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影响因子：
--
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DOI：
10.1210/jcem.81.3.8772613
发表时间：
1996
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
--
作者：
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DOI：
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发表时间：
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期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
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通讯作者：
Yen,SS
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DOI：
10.1152/ajpheart.1996.271.5.h1999
发表时间：
1996
期刊：
The American journal of physiology.
影响因子：
--
作者：
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通讯作者：
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