Upstream regulatory regions controlling the expression of the yeast maltase gene.
Upstream regulatory regions controlling the expression of the yeast maltase gene.
复制标题
控制酵母麦芽糖酶基因表达的上游调控区。
DOI：
10.1128/mcb.7.7.2477-2483.1987
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发表时间：
1987
影响因子：
5.3
通讯作者：
Marmur,J
中科院分区：
文献类型：
--
作者：
Hong,SH;Marmur,J
麦芽糖酶（maltase， MALS）和麦芽糖渗透酶（MALT， MALT）基因在转录水平上相互调控；它们是由麦芽糖在正作用调节（MALR）基因和葡萄糖抑制的碳分解代谢物的存在下协调诱导的。我们在theMAL6Sgene的上游区域产生了一系列缺失，以详细检查调控元件。结果表明，当翻译起始密码子上游320 ~ 380个碱基对之间的区域被删除时，麦芽糖诱导表达丢失。该区域包含一个不完美的反向重复序列（-361至-327）或4个短的直接重复序列，可能作为麦尔塔酶基因上游激活位点（UASM）的组成部分，或两者兼有。当一段富含t的序列（-253到-237）被删除时，麦芽糖酶基因对碳分解代谢抑制的易感性受到影响。
The expression of the maltase (MALS) and the maltose permease (MALT) genes in Saccharomyces species is coregulated at the transcriptional level; they are coordinately induced by maltose in the presence of a positively acting regulatory (MALR) gene and carbon catabolite repressed by glucose. We generated a series of deletions in the upstream region of theMAL6Sgene to examine the regulatory elements in detail. The results showed that inducible expression by maltose was lost when the region between 320 and 380 base pairs upstream of the translation initiation codon was deleted. This region contained an imperfect inverted repeat sequence (-361 to -327) or four copies of short direct repeats that might serve as components of the upstream activation site (UASM) for the maltase gene, or both. When a stretch of T-rich sequence (-253 to -237) was deleted, the susceptibility of the maltase gene to carbon catabolite repression was affected.