Activation of A1 and A2 noradrenergic neurons in response to running in the rat
Activation of A1 and A2 noradrenergic neurons in response to running in the rat
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DOI：
10.1016/j.neulet.2005.10.053
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发表时间：
2006-02-27
影响因子：
2.5
通讯作者：
Soya, H
中科院分区：
文献类型：
--
作者：
Ohiwa, N;Saito, T;Soya, H
由于跑步伴随血乳酸积累刺激促肾上腺皮质激素(ACTH)的释放，超过乳酸阈值(LT)的跑步就是应激(Running Stress)。为了观察投射到下丘脑的A1/A2去甲肾上腺素能神经元在跑步应激条件下是否被激活，采用c-Fos免疫组织化学方法比较了跑步应激对A1/A2去甲肾上腺素能神经元的影响。跑步应激组的血乳酸和血浆ACTH浓度显著升高，而无应激反应组和对照组的血乳酸和血浆ACTH浓度无明显变化，证实了不同强度的跑步有无应激对机体的生理影响不同。运动应激显著增加A1/A2去甲肾上腺素能神经元c-Fos的积聚。无应激运动时，A1/A2去甲肾上腺素能神经元c-Fos表达显著增加，且增加的百分比小于运动应激组。A1/A2去甲肾上腺素能神经元中c-Fos的表达程度与运动强度有关，表明这种神经元的激活是速度依赖性的。因此，我们认为A1/A2去甲肾上腺素能神经元的激活不仅是对跑步应激的反应，也是对其他生理性跑步的反应，而非应激跑则增强了去甲肾上腺素能神经元。这些发现将有助于对特定神经回路的研究，并有助于确定它们在不同强度跑步时的功能。(C)2005爱思唯尔爱尔兰有限公司。保留所有权利。
Since running accompanied with blood lactate accumulation stimulates the release of adrenocorticotropic hormone (ACTH), running above the lactate threshold (LT) acts as stress (running stress). To examine whether A1/A2 noradrenergic neurons that project to the hypothalamus activate under running stress, c-Fos immunohistochemistry was used to compare the effects of running with or without stress response on A1/A2 noradrenergic neurons. Blood lactate and plasma ACTH concentrations significantly increased in the running stress group, but not in the running without stress response and control groups, confirming different physiological impacts between different intensity of running with or without stress. Running stress markedly increased c-Fos accumulation in the A1/A2 noradrenergic neurons. Running without stress response also induced a significant increase in c-Fos expression in the A1/A2 noradrenergic neurons, and the percentage of the increase was smaller than that of running stress. The extent of c-Fos expression in the A1/A2 noradrenergic neurons correlates with exercise intensity, signifying that this neuronal activation is running speed-dependent. We thus suggest that A1/A2 noradrenergic neurons are activated in response to not only running stress, but also to other physiological running, enhanced by non-stressful running. These findings will be helpful in studies of specific neurocircuits and in identifying their functions in response to running at different intensities. (C) 2005 Elsevier Ireland Ltd. All rights reserved.