Mitonuclear mismatch alters performance and reproductive success in naturally introgressed populations of a montane leaf beetle
Mitonuclear mismatch alters performance and reproductive success in naturally introgressed populations of a montane leaf beetle
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DOI：
10.1111/evo.13962
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发表时间：
2020-04-27
期刊：
影响因子：
3.3
通讯作者：
Dahlhoff，Elizabeth P.
中科院分区：
文献类型：
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作者：
Rank，Nathan E.;Mardulyn，Patrick;Dahlhoff，Elizabeth P.
核基因组和线粒体基因组之间的协调对于动物适应当地环境的能力所涉及的代谢过程至关重要，然而，在线粒体和核基因组存在差异的个体自然杂交的种群中，很少研究有丝分裂相互作用的后果。在加利福尼亚州的内华达山脉，沿着纬度温度梯度评估了叶甲叶甲的遗传变异。线粒体细胞色素氧化酶II(COII)和核基因磷酸葡萄糖异构酶(PGI)的变异表现出一致性，并在65公里的样带上显著大于其他10个座位。利用中性基因座的结构分析确定了一个南方和北方的亚种群，它们在中央排水毕晓普河进行杂交。COII和PGI被用来作为线粒体和核遗传变异的指示器，对来自该混合种群的甲虫进行了田间和实验室实验。地理上“匹配”的甲虫的繁殖力、幼虫发育率、奔跑速度和雄性交配频率均高于“不匹配”的有丝分裂核型。对于拥有南方线粒体的北方核型个体来说，有丝分裂核错配的影响最大，在热处理或高海拔地区最明显。这些发现表明，有丝分裂不相容在自然界中会降低性能和生殖成功，这种影响在极端环境下可能会加剧。
Coordination between nuclear and mitochondrial genomes is critical to metabolic processes underlying animals' ability to adapt to local environments, yet consequences of mitonuclear interactions have rarely been investigated in populations where individuals with divergent mitochondrial and nuclear genomes naturally interbreed. Genetic variation in the leaf beetleChrysomela aeneicolliswas assessed along a latitudinal thermal gradient in California's Sierra Nevada. Variation at mitochondrialcytochrome oxidaseII(COII) and the nuclear genephosphoglucose isomerase(PGI) shows concordance and was significantly greater along a 65 km transect than 10 other loci. STRUCTURE analyses using neutral loci identified a southern and northern subpopulation, which interbreed in the central drainage Bishop Creek.COIIandPGIwere used as indicators of mitochondrial and nuclear genetic variation in field and laboratory experiments conducted on beetles from this admixed population. Fecundity, larval development rate, running speed and male mating frequency were higher for beetles with geographically “matched” than “mismatched” mitonuclear genotypes. Effects of mitonuclear mismatch were largest for individuals with northern nuclear genotypes possessing southern mitochondria and were most pronounced after heat treatment or at high elevation. These findings suggest that mitonuclear incompatibility diminishes performance and reproductive success in nature, effects that could intensify at environmental extremes.