Screening and characterization of a phosphopentomutase useful for enzymatic production of 2′-deoxyribonucleoside
Screening and characterization of a phosphopentomutase useful for enzymatic production of 2′-deoxyribonucleoside
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DOI：
10.1016/j.nbt.2009.03.015
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发表时间：
2009-10-01
影响因子：
5.4
通讯作者：
Shimizu, Sakayu
中科院分区：
文献类型：
--
作者：
Horinouchi, Nobuyuki;Kawano, Takako;Shimizu, Sakayu
发现球形芽孢杆菌AKU 229产生用于酶促2 ′-脱氧核糖核苷生产的耐乙酰丙酮和耐磷酸化化合物的磷酸变位酶。在大肠杆菌中克隆并表达了编码磷酸戊变位酶的基因。急诊大肠杆菌表达B.对于在乙醛和磷酸化化合物如果糖1，6-二磷酸和D-甘油醛3-磷酸存在下生产2 ′-脱氧核糖核苷，sphaericus磷酸变位酶是极好的催化剂，磷酸化化合物通过与酵母细胞的糖酵解从葡萄糖衍生，并且大量存在于用于酶促2 ′-脱氧核糖核苷生产的实际反应混合物中。
Bacillus sphaericus AKU 229 was found to produce an acetaldehyde-tolerant and phosphorylated compound-tolerant phosphopentomutase useful for enzymatic 2'-deoxyribonucleoside production. The gene encoding the phosphopentomutase was cloned and expressed in Escherichia coli. The E. coli expressing B. sphaericus phosphopentomutase was an excellent catalyst as to production of 2'-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds such as fructose 1,6-diphosphate, and D-glyceraldehyde 3-phosphate, which are derived from glucose through glycolysis with yeast cells, and exist abundantly in the practical reaction mixture for enzymatic 2'-deoxyribonucleoside production.