Evaluation of biocompatibility of the surface of polyethylene films modified with various water soluble polymers using Ar plasma-post polymerization technique
Evaluation of biocompatibility of the surface of polyethylene films modified with various water soluble polymers using Ar plasma-post polymerization technique
复制标题
DOI：
10.1002/1439-2054(20001001)282:1
复制
发表时间：
2000-10
影响因子：
3.9
通讯作者：
K. Sugiyama;Takako Matsumoto;Yuki Yamazaki
中科院分区：
文献类型：
--
作者：
K. Sugiyama;Takako Matsumoto;Yuki Yamazaki
用水溶性聚合物如聚[2-（2-甲基丙烯酰氧基）苯基]丙烯酰胺（poly [2-（2-methylphenyl）phenyl）-2-methylphenyl）（poly[2-（2-methylphenyl）phenyl）-2-methylphenyl）丙烯酰胺（poly[2-（2-methylphenyl）phenyl] phenyl）表面改性的聚乙烯（g-PE）、PMPC-g-PE、PGEMA-g-PE、PNIPAAm-g-PE和PHPMA-g-PE膜。（甲基丙烯酰氧基）乙基磷酰胆碱]（PMPC），聚[2-甲基丙烯酸（葡糖基氧基）乙酯]（PGEMA），聚（N-异丙基丙烯酰胺）（PNIPAAm）和聚[N-采用Ar等离子体后聚合技术，通过接枝共聚制备了2-（2-羟丙基）甲基丙烯酰胺（PHPMA）。用X射线光电子能谱仪对接枝膜的表面进行了表征，接枝率分别为5.31%、2.83%和3.40%。通过对血清蛋白的吸附和凝血酶与合成底物S-2238在g-PE膜存在下的酶促反应的米氏常数（Km）来评价g-PE膜的生物相容性。PMPC、聚氧乙烯（POE）、PGEMA、PNIPAAm和PHPMA等水溶性聚合物的生物相容性也通过凝血酶与聚合物溶液中S-2238的相同酶促反应来评估。在水溶性聚合物存在下，Km值按PMPC > POE > PGEMA > PNIPAAm > PHPMA的顺序降低。结果表明，PMPC-g-PE膜的生物相容性最好，其表面吸附蛋白质的量最少，酶促反应的Km值最大。
Surface modified polyethylene (g-PE), PMPC-g-PE, PGEMA-g-PE, PNIPAAm-g-PE and PHPMA-g-PE films with the water soluble polymers such as poly[2-(methacryloyloxy)ethyl phosphorylcholine] (PMPC), poly[2-(glucosyloxy)ethyl methacrylate] (PGEMA), poly(N-isopropylacrylamide) (PNIPAAm) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) were prepared by graft copolymerization using an Ar plasma-post polymerization technique. The surface of the g-PE films was characterized by means of X-ray photoelectron spectroscopy and the grafting percentage of PMPC, PNIPAAm and PHPMA was found to be 5.31, 2.83, and 3.40% for the corresponding g-PE film. Biocompatibility of the g-PE films was evaluated by the adsorption of serum proteins and the Michaelis constant (K m ) for the enzymatic reaction of thrombin with synthetic substrate S-2238 in the presence of g-PE film. The biocompatibility of water soluble polymers such as PMPC, polyoxyethylene (POE), PGEMA, PNIPAAm and PHPMA was also evaluated by the same enzymatic reaction of thrombin with S-2238 in their polymer solutions. The K m values in the presence of water soluble polymers was found to decrease in the order PMPC > POE > PGEMA > PNIPAAm > PHPMA. As a conclusion, PMPC-g-PE film exhibited the most biocompatibility among g-PE films because its surface adsorbed less protein than those of the untreated PE and other g-PE films and it showed the largest K m for the enzymatic reaction.