Immunization of calves against the microfilariae of Onchocerca lienalis
Immunization of calves against the microfilariae of Onchocerca lienalis
复制标题
犊牛抗盘尾丝虫微丝蚴的免疫接种
DOI：
10.1017/s0022149x00034684
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发表时间：
1982
影响因子：
1.6
通讯作者：
A. Bianco
中科院分区：
文献类型：
--
作者：
S. Townson;A. Bianco
通过皮下注射悬浮在磷酸盐缓冲盐水中的超声处理的Onchocerca lienalis微丝蚴对三只泽西公牛小牛进行免疫，并且三只对照动物仅注射培养基。所有小牛在27天后接受加强注射，并在加强注射后44天用活微丝蚴攻毒。攻毒后，仅免疫动物出现循环嗜酸性粒细胞水平升高。在攻毒后10天进行尸检时，与攻毒对照组相比，免疫动物的微丝蚴回收率降低了97%。在人类盘尾丝虫病中，微丝蚴是主要的病理原因，我们认为对O。在天然牛宿主和近交系啮齿动物中的lienalis为研究对盘尾丝虫微丝蚴的免疫提供了一个有希望的模型。
ABSTRACT Three Jersey bull calves were immunized by subcutaneous injections of sonicated Onchocerca lienalis microfilariae suspended in phosphate buffered saline, and three control animals were injected with medium only. All calves received booster injections 27 days later, and were challenged with live microfilariae 44 days after the booster. Following challenge, only the immunized animals developed an elevated level of circulating eosinophils. When necropsied ten days after challenge there was a 97% reduction in recoveries of microfilariae from immunized animals compared to challenge controls. In human onchocerciasis it is the microfilariae which are the principal cause of pathology, and we believe that studies on O. lienalis in both the natural bovine host and in inbred rodents provide a promising model to investigate immunity to Onchocerca microfilariae.