Two sox9 genes on duplicated zebrafish chromosomes:: Expression of similar transcription activators in distinct sites
Two sox9 genes on duplicated zebrafish chromosomes:: Expression of similar transcription activators in distinct sites
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DOI：
10.1006/dbio.2000.0129
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发表时间：
2001-03-01
影响因子：
2.7
通讯作者：
Chung, BC
中科院分区：
文献类型：
--
作者：
Chiang, EFL;Pai, CI;Chung, BC
Sox 9是哺乳动物中软骨形成和睾丸决定所需的转录因子。我们已经从斑马鱼中克隆了两个sox 9基因，分别命名为sox 9a和sox 9 b。基因同源性分析表明，这两个基因都与四足动物的SOX 3基因同源。遗传作图表明，这两个位点位于染色体片段，显然是在一个大规模的基因组复制事件中复制的鳐鳍鱼的胚胎发育。Sox 9a和Sox 9 b蛋白在体外均与HMG共有DNA序列结合。我们测试了不同结构域的反式激活潜力，并确定了一个潜在的激活域位于中间的Sox 9a和Sox 9 b。在胚胎发育过程中，sox 9a和sox 9 b的表达模式是不同的，但在大脑、头部骨骼和鳍的某些区域重叠。sox 9a/B的表达与软骨形成元件中的col 2 a1的表达具有良好的相关性。在成体中，sox 9a在许多组织中表达，包括脑、肌肉、鳍和睾丸，而sox 9 b的表达仅限于卵巢的卵黄发生前卵母细胞。这种表达模式预示sox 9a和sox 9 b可能在发育过程中的某些特定组织中具有独特的功能。基因复制的发育基因功能的进化的作用进行了讨论。(C)北京：科学出版社.
Sox9 is a transcription factor required for cartilage formation and testis determination in mammals. We have cloned from zebrafish two sox9 genes, termed sox9a and sox9b. Gene phylogenies showed that both genes are orthologous to tetrapod SOX3 genes. Genetic mapping showed that these two loci reside on chromosome segments that were apparently duplicated in a large-scale genomic duplication event in ray fin fish phylogeny. Both Sox9a and Sox9b proteins bind to the HMG consensus DNA sequences in vitro. We tested different domains for transactivation potential and identified a potential activation domain located in the middle of both Sox9a and Sox9b. During embryogenesis, sox9a and sox9b expression patterns are distinct but overlap in some regions of the brain, head skeleton, and fins. Expression of sox9a/b correlates well with that of col2a1 in chondrogenic elements. In the adults, sox9a is expressed in many tissues including brain, muscle, fin, and testis, whereas sox9b expression is restricted to previtellogenic oocytes of the ovary. This expression pattern predicts that sox9a and sox9b may have unique functions in some specific tissues during development. The role of gene duplication for the evolution of developmental gene function is discussed. (C) 2001 Academic Press.