Biomarker Mapping on Skin Tape Strips Using MALDI Mass Spectrometry Imaging
Biomarker Mapping on Skin Tape Strips Using MALDI Mass Spectrometry Imaging
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DOI：
10.1007/s13361-019-02277-5
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发表时间：
2019-10-01
影响因子：
3.2
通讯作者：
Stamatas, Georgios N.
中科院分区：
文献类型：
--
作者：
Hochart, Guillaume;Bonnel, David;Stamatas, Georgios N.
角质形成细胞的组织和生物化学在形成皮肤的保护屏障方面很重要。内在和外在因素可在细胞和分子水平上影响皮肤屏障功能。基质辅助激光解吸/电离（MALDI）质谱成像，一种结合分子方面和组织学细节的技术，已被证明是一种有价值的方法，在包括药理学，皮肤病学和美容学在内的各个学科。它通常需要在冷冻组织切片后制备的离体样品。本文论证了对非侵入性皮肤上收集的胶带条进行MALDI分析的可行性。其目的是获得分子成像的角质细胞对磁带上的新的生物学见解。从人类志愿者的两个皮肤部位（掌侧前臂和脸颊）收集胶带。10种与皮肤屏障功能相关的分子用7 T MALDI傅里叶变换离子回旋共振（FTICR）仪器以高空间分辨率的单一采集模式检测。该方法的灵敏度足以创建分子图谱，该图谱可以覆盖在胶带相同区域的透射显微镜图像上。对两个皮肤部位胶带的分子分布分析与两个部位的已知皮肤特性一致，证实了观察结果的有效性。使用系统聚类分析来区分角质细胞群体的基础上，他们的分子概况。此外，形态学分析提供了一种新的方式来考虑同一胶带上的角质细胞的统计群体，而不是测量单个平均值，提供了与其结构-功能关系相关的额外有用信息。
Keratinocyte organization and biochemistry are important in forming the skin's protective barrier. Intrinsic and extrinsic factors can affect skin barrier function at the cellular and molecular levels. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging, a technique which combines both molecular aspects and histological details, has proven to be a valuable method in various disciplines including pharmacology, dermatology and cosmetology. It typically requires ex vivo samples, prepared following frozen tissue sectioning. This paper demonstrates the feasibility of performing MALDI analysis on tape strips collected non-invasively on skin. The aim is to obtain molecular imaging of corneocytes on tapes towards novel biological insights. Tapes were collected from two skin sites (volar forearm and cheek) of human volunteers. Ten molecules relating to skin barrier function were detected with a single mode of acquisition at high spatial resolution with a 7 T MALDI-Fourier transform ion cyclotron resonance (FTICR) instrument. The method sensitivity was adequate to create molecular maps which could be overlaid on transmission microscopy images of the same area of the tape. Analysis of the molecular distributions from tapes at the two skin sites was consistent with the known skin properties of the two sites, confirming the validity of the observations. Hierarchical clustering analysis was used to differentiate corneocyte populations based on their molecular profiles. Furthermore, morphological analysis provided a new way of considering statistical populations of corneocytes on the same tape, rather than measuring a single averaged value, providing additional useful information relating to their structure-function relationship.