A Bacillus subtilis strain can reduce hexavalent chromium to trivalent and an nfrA gene is involved
A Bacillus subtilis strain can reduce hexavalent chromium to trivalent and an nfrA gene is involved
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枯草芽孢杆菌菌株可将六价铬还原为三价，涉及 nfrA 基因
DOI：
10.1016/j.ibiod.2014.10.017
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发表时间：
2015-01-01
影响因子：
4.8
通讯作者：
Li, Xiangkai
中科院分区：
文献类型：
--
作者：
Zheng, Zhe;Li, Yabo;Li, Xiangkai
白银市位于黄河上游，甘肃省中部，重金属铬污染严重。从甘肃白银某稀土矿中分离到一株能将六价铬（Cr（VI））好氧还原为三价铬（Cr（III））的革兰氏阳性菌BYCr-1。16 SrRNA分析表明，该菌株与枯草芽孢杆菌（Bacillussubtilis）亲缘关系较近。在M9培养基中孵育48 h后，可将0.2 mM Cr（VI）还原为Cr（III）。透射电子显微镜（TEM）图像显示，Cr（III）沉淀物位于细胞内外。Cr（VI）处理后nfrA基因表达上调53倍。coli-NfrA表现出升高的铬还原能力。我们的研究结果表明，BYCr-1是能够抵抗和减少高浓度的Cr（VI），这使得它成为一个潜在的合适的候选人的Cr（VI）污染的生物修复。该研究还揭示了nfrA赋予枯草芽孢杆菌中的Cr（VI）还原能力。(C)2014爱思唯尔有限公司版权所有。
Baiyin city, which is located upstream of the Yellow River and in the central part of Gansu province, China, is severely contaminated by heavy metals such as chromium. A Gram-positive bacterium BYCr-1 capable of reducing hexavalent chromium (Cr(VI)) to trivalent (Cr(III)) aerobically was isolated from a rare-earth ore in Baiyin, Gansu, China. 16S rRNA analysis revealed that it was closely related to Bacillus subtilis. It can reduce 0.2 mM Cr(VI) to Cr(III) in M9 medium after 48 h incubation. Transmission electron microscopy (TEM) image showed that Cr(III) precipitates were located both inside and outside the cells. An nfrA gene was upregulated by 53 folds upon Cr(VI) treatment Furthermore, E. coli-NfrA demonstrated elevated chromate-reducing ability. Our results indicate that BYCr-1 is able to resist and reduce high concentrations of Cr(VI), which makes it a potentially suitable candidate for bioremediation of Cr(VI) contamination. This study also reveals that nfrA confers Cr(VI)-reducing ability in Bacillus subtilis. (C) 2014 Elsevier Ltd. All rights reserved.