The commercial pig as a model of spontaneously-occurring osteoarthritis
The commercial pig as a model of spontaneously-occurring osteoarthritis
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DOI：
10.1186/s12891-019-2452-0
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发表时间：
2019-02-11
影响因子：
2.3
通讯作者：
Jones, Simon W.
中科院分区：
文献类型：
--
作者：
Macfadyen, Mhairi A.;Daniel, Zoe;Jones, Simon W.
研究背景自发性损伤的临床前骨关节炎模型可以更好地反映人类骨关节炎的发生和发展。目的是评估商业猪作为一个模型的自发性骨关节炎的发展，通过检查疼痛相关的行为，关节软骨的完整性，以及使用猪软骨外植体和分离的软骨细胞和成骨细胞的体外和体外study.MethodsFemale猪（大白色x长白猪x杜洛克）进行了检查，在不同的年龄从6周到3- 4岁。跛行被评估为疼痛相关行为的标志。通过软骨病评分和蛋白多糖组织学染色确定股胫关节软骨完整性。通过ELISA和DMMB测定评估软骨外植体和原代猪软骨细胞中的IL-6产生和蛋白聚糖降解。主要的猪成骨细胞从受损和未受损的关节，软骨病评分确定，进行了评估矿化，增殖和线粒体功能作为一个标记的代谢capability.Results80周及以上的猪表现出跛行。股骨髁和胫骨平台软骨的骨关节炎病变从40周开始明显，并随着年龄的增加而加重，直到3- 4岁。受损关节的软骨表现出蛋白多糖丢失，这与软骨病评分呈正相关。用IL-1或内脂素刺激猪软骨外植体和原代软骨细胞诱导IL-6产生和蛋白聚糖降解。从受损关节的主要猪成骨细胞表现出减少增殖，矿化，和代谢capability.ConclusionIn结论，商业猪自发性骨关节炎的替代模型和优良的组织来源，在体外和体外研究。
BackgroundPreclinical osteoarthritis models where damage occurs spontaneously may better reflect the initiation and development of human osteoarthritis. The aim was to assess the commercial pig as a model of spontaneous osteoarthritis development by examining pain-associated behaviour, joint cartilage integrity, as well as the use of porcine cartilage explants and isolated chondrocytes and osteoblasts for ex vivo and in vitro studies.MethodsFemale pigs (Large white x Landrace x Duroc) were examined at different ages from 6weeks to 3-4years old. Lameness was assessed as a marker of pain-associated behaviour. Femorotibial joint cartilage integrity was determined by chondropathy scoring and histological staining of proteoglycan. IL-6 production and proteoglycan degradation was assessed in cartilage explants and primary porcine chondrocytes by ELISA and DMMB assay. Primary porcine osteoblasts from damaged and non-damaged joints, as determined by chondropathy scoring, were assessed for mineralisation, proliferative and mitochondrial function as a marker of metabolic capacity.ResultsPigs aged 80weeks and older exhibited lameness. Osteoarthritic lesions in femoral condyle and tibial plateau cartilage were apparent from 40weeks and increased in severity with age up to 3-4years old. Cartilage from damaged joints exhibited proteoglycan loss, which positively correlated with chondropathy score. Stimulation of porcine cartilage explants and primary chondrocytes with either IL-1 or visfatin induced IL-6 production and proteoglycan degradation. Primary porcine osteoblasts from damaged joints exhibited reduced proliferative, mineralisation, and metabolic capacity.ConclusionIn conclusion, the commercial pig represents an alternative model of spontaneous osteoarthritis and an excellent source of tissue for in vitro and ex vivo studies.