VAMP711 Is Required for Abscisic Acid-Mediated Inhibition of Plasma Membrane H+-ATPase Activity
VAMP711 Is Required for Abscisic Acid-Mediated Inhibition of Plasma Membrane H+-ATPase Activity
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VAMP711 是脱落酸介导的质膜 H-ATP 酶活性抑制所必需的
DOI：
10.1104/pp.18.00499
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发表时间：
2018
期刊：
影响因子：
7.4
通讯作者：
Guo Yan
中科院分区：
文献类型：
--
作者：
Xue Yuan;Yang Yongqing;Yang Zhijia;Wang Xiangfeng;Guo Yan
干旱胁迫是影响植物生长发育的限制性环境因子。植物激素脱落酸（阿坝）在植物干旱响应中起着重要作用。前期研究表明，阿坝抑制质膜H+-ATPase（PM H+-ATPase）活性，PM H+-ATPase活性的降低促进了干旱胁迫下气孔的关闭，从而减少水分损失。然而，其潜在的分子机制还不清楚。在这项研究中，我们发现，在拟南芥（拟南芥），阿坝诱导的N-乙基马来酰亚胺敏感因子附着蛋白受体蛋白，即囊泡相关膜蛋白711（VAMP 711），与拟南芥PM H+-ATP酶AHA 1和AHA 2相互作用。这种相互作用发生在它们的C-末端，并抑制PM H+-ATP酶活性。VAMP 711基因缺失导致拟南芥质膜H+-ATP酶活性升高，气孔关闭速度减慢。此外，VAMP 711的过表达部分地挽救了fost 2 -2D的干旱敏感表型，fost 2 -2D是AHA 1中的突变，导致组成性激活PM H+-ATP酶。我们的研究结果表明，VAMP 711参与调节ABA介导的抑制PM H+-ATP酶活性和气孔关闭响应干旱胁迫。
Drought stress is a limiting environmental factor that affects plant growth and development. The plant hormone abscisic acid (ABA) plays an important role in plant drought responses. Previous studies have indicated that ABA inhibits plasma membrane H+-ATPase (PM H+-ATPase) activity, and the decrease in PM H+-ATPase activity promotes stomatal closure under drought stress, thereby reducing water loss. However, the underlying molecular mechanisms are not well understood. In this study, we found that in Arabidopsis (Arabidopsis thaliana), ABA induces anN-ethylmaleimide-sensitive factor attachment protein receptor protein, namely, VESICLE-ASSOCIATED MEMBRANE PROTEIN 711 (VAMP711), to interact with the Arabidopsis PM H+-ATPases AHA1 and AHA2. The interaction occurs at their C-termini and inhibits PM H+-ATPase activity. Deletion ofVAMP711in Arabidopsis results in a higher PM H+-ATPase activity and slower stomatal closure in response to ABA and drought treatments. In addition, overexpression ofVAMP711partially rescues the drought-sensitive phenotype ofost2-2D, a mutation inAHA1resulting in a constitutive activated PM H+-ATPase. Our results demonstrate that VAMP711 is involved in regulating ABA-mediated inhibition of PM H+-ATPase activity and stomatal closure in response to drought stress.