Distribution and Genetic Profiles of Campylobacter in Commercial Broiler Production from Breeder to Slaughter in Thailand.
Distribution and Genetic Profiles of Campylobacter in Commercial Broiler Production from Breeder to Slaughter in Thailand.
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DOI：
10.1371/journal.pone.0149585
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Luangtongkum T
中科院分区：
文献类型：
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作者：
Prachantasena S;Charununtakorn P;Muangnoicharoen S;Hankla L;Techawal N;Chaveerach P;Tuitemwong P;Chokesajjawatee N;Williams N;Humphrey T;Luangtongkum T
家禽和家禽产品通常被认为是世界范围内人类感染弯曲杆菌的主要媒介。为了减少人类病例的数量，必须更好地了解家禽中弯曲杆菌的流行病学。因此，本研究的目的是确定弯曲杆菌在泰国鸡肉生产行业中的分布及其遗传关联性。在2012年6月至10月期间，按时间顺序调查了五个肉鸡生产链的整个肉鸡生产流程(即种鸡群、孵化场、肉鸡养殖场和屠宰场)。对各生产阶段的空肠弯曲菌代表菌株进行了FLAA、SVR测序和多位点序列分型。在311株分离物中，共鉴定出29个FlaA SVR等位基因和17个序列类型(STS)。本研究中发现的常见克隆复合体为CC-45、CC-353、CC-354和CC-574。从养殖户分离到的空肠弯曲菌与从肉鸡和肉鸡屠体中分离到的空肠弯曲菌有较远的亲缘关系，而从屠宰场环境和肉制品中分离到的空肠弯曲菌与从肉鸡群中分离到的空肠弯曲菌相似。屠宰场空肠弯曲菌的基因分型鉴定表明，肉鸡是鸡肉加工过程中弯曲菌污染的主要来源。为了有效地减少禽肉产品中的弯曲杆菌，控制和预防战略应针对农场和屠宰场两个层面。
Poultry and poultry products are commonly considered as the major vehicle of Campylobacter infection in humans worldwide. To reduce the number of human cases, the epidemiology of Campylobacter in poultry must be better understood. Therefore, the objective of the present study was to determine the distribution and genetic relatedness of Campylobacter in the Thai chicken production industry. During June to October 2012, entire broiler production processes (i.e., breeder flock, hatchery, broiler farm and slaughterhouse) of five broiler production chains were investigated chronologically. Representative isolates of C. jejuni from each production stage were characterized by flaA SVR sequencing and multilocus sequence typing (MLST). Amongst 311 selected isolates, 29 flaA SVR alleles and 17 sequence types (STs) were identified. The common clonal complexes (CCs) found in this study were CC-45, CC-353, CC-354 and CC-574. C. jejuni isolated from breeders were distantly related to those isolated from broilers and chicken carcasses, while C. jejuni isolates from the slaughterhouse environment and meat products were similar to those isolated from broiler flocks. Genotypic identification of C. jejuni in slaughterhouses indicated that broilers were the main source of Campylobacter contamination of chicken meat during processing. To effectively reduce Campylobacter in poultry meat products, control and prevention strategies should be aimed at both farm and slaughterhouse levels.