Early Fish Myoseptal Cells: Insights from the Trout and Relationships with Amniote Axial Tenocytes
Early Fish Myoseptal Cells: Insights from the Trout and Relationships with Amniote Axial Tenocytes
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DOI：
10.1371/journal.pone.0091876
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发表时间：
2014-03-12
期刊：
影响因子：
3.7
通讯作者：
Rescan, Pierre-Yves
中科院分区：
文献类型：
--
作者：
Bricard, Yoann;Ralliere, Cecile;Rescan, Pierre-Yves
鱼的躯干肌肉是由一系列纵向的肌群组成的，这些肌群由一片被称为肌隔膜的结缔组织隔开，肌纤维附着在这些结缔组织上。在本研究中，我们在鲑鱼中发现，分隔两个体节的肌隔最初是无细胞的，由纤维连接蛋白、层粘连蛋白和I型胶原等物质组成，但在体细胞发生完成后，在相邻的肌节之间观察到肌间隔细胞与围绕轴向结构的骨骼细胞形成连续体。肌间隔细胞不表达Pax3、Pax7和mygenin等肌源性标志物，但表达多种肌腱相关胶原，包括Col1a1、Col5a2和Col12a1，以及参与基质重塑的分泌分子--血管生成素样7。以col1a1为标记基因，我们在鲑鱼胚胎发育过程中观察到了向肌节腹侧扩散细胞的初步标记。后来，发现标记细胞更多地在背部环绕脊索或侵入肌间间隙。这开启了这样一种可能性，即硬骨节不仅可以像以前报道的那样分化为骨源性间充质细胞，还可以分化为肌间充质细胞。我们进一步表明，肌间隔细胞不同于脊索周围的骨骼细胞，这是由于硬化轴的特殊表达，硬化轴是羊膜中肌腱细胞的独特标志。综上所述，肌间隔细胞的位置、分子特征和可能的硬化症起源表明，鱼的肌间隔细胞与羊膜中轴向腱细胞同源。
The trunk muscle in fish is organized as longitudinal series of myomeres which are separated by sheets of connective tissue called myoseptum to which myofibers attach. In this study we show in the trout that the myoseptum separating two somites is initially acellular and composed of matricial components such as fibronectin, laminin and collagen I. However, myoseptal cells forming a continuum with skeletogenic cells surrounding axial structures are observed between adjacent myotomes after the completion of somitogenesis. The myoseptal cells do not express myogenic markers such as Pax3, Pax7 and myogenin but express several tendon-associated collagens including col1a1, col5a2 and col12a1 and angiopoietin-like 7, which is a secreted molecule involved in matrix remodelling. Using col1a1 as a marker gene, we observed in developing trout embryo an initial labelling in disseminating cells ventral to the myotome. Later, labelled cells were found more dorsally encircling the notochord or invading the intermyotomal space. This opens the possibility that the sclerotome gives rise not only to skeletogenic mesenchymal cells, as previously reported, but also to myoseptal cells. We furthermore show that myoseptal cells differ from skeletogenic cells found around the notochord by the specific expression of Scleraxis, a distinctive marker of tendon cells in amniotes. In conclusion, the location, the molecular signature and the possible sclerotomal origin of the myoseptal cells suggest that the fish myoseptal cells are homologous to the axial tenocytes in amniotes.