Screening for antioxidant activity in edible plant products: Comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay
Screening for antioxidant activity in edible plant products: Comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay
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DOI：
10.1021/jf035372g
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发表时间：
2004-04-21
影响因子：
6.1
通讯作者：
Yamane, Y
中科院分区：
文献类型：
--
作者：
Katsube, T;Tabata, H;Yamane, Y
低密度脂蛋白（LDL）的氧化与动脉粥样硬化形成有关。防止LDL氧化的抗氧化剂可以减少动脉粥样硬化。本研究调查了可食用植物产品中的LDL抗氧化活性，以开发预防动脉粥样硬化的膳食补充剂。用70%乙醇水溶液提取52种食用植物，以氧化滞后时间为指标，测定其抗氧化活性，并以表没食子儿茶素没食子酸酯当量表示。测定了1，1-二苯基-2-苦肼基（DPPH）自由基清除活性和总酚含量，并与LDL的抗氧化活性进行了比较。LDL氧化活性最高的植物产品是赤桐叶、女贞叶、绿色茶[Camellia sinensis（L.）O. Kuntze]和涩柿（Diospyros kaki）。本研究揭示了植物产品中高水平的LDL抗氧化活性，这种活性水平在DPPH自由基清除试验和Folin-Ciocalteu试验中被低估。
Oxidation of low-density lipoprotein (LDL) has been implicated in atherogenesis. Antioxidants that prevent LDL from oxidizing may reduce atherosclerosis. This study investigated LDL antioxidant activity in edible plant products for development of dietary supplementation to prevent atherosclerosis. Fifty-two kinds of edible plants were extracted using 70% aqueous ethanol solution, and the antioxidant activity of the extracts, which inhibit human LDL oxidation induced by copper ion, was determined on the basis of the oxidation lag time and represented as epigallocatechin 3-gallate equivalent. 1,1Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and total phenolic content were also measured for comparisons with antioxidant activity in LDL. Plant products showing the greatest activity in LDL oxidation assay were akamegashiwa (Mallotus japonicus) leaf, Japanese privet (Ligustrum japonicum) leaf, green tea [Camellia sinensis (L.) O. Kuntze], and astringent persimmon (Diospyros kaki). The present study revealed high levels of LDL antioxidant activity in plant products for which such activity levels are underestimated in the DPPH radical scavenging assay and Folin-Ciocalteu assay.